简介:为了探索狼发情行为的生理基础,2005年12月至2006年4月,在哈尔滨北方森林动物园对参与繁殖的四只雌性狼采用非损伤性方法收集动物粪便。通过粪样激素抽提,利用化学衍生技术,对抽提样品进行衍生,采用高效液相色谱分析法(HPLC),成功实现对狼粪样中的类固醇激素进行分离,结果表明:雌狼发情前雌二醇(E2)的含量一直处于较低水平,进入发情后含量会迅速升高至峰值且发情期间在较高的水平波动,这与多数哺乳动物发情期E2出现峰值的现象基本一致,峰值的出现即是雌狼发情的高潮期的来临。发情期E2浓度显著高于非发情期(p〈0.01),P浓度在整个孕期也显著升高(P〈0.01),四只雌狼均成功与配对的雄狼发生交配现象,并在当繁殖季节成功产仔。
简介:Thispaperwastoinvestigatebasicpropertiesofmicro/nanofibrilsfrompoplarwithahighpressurehomogenizer.Theirbasicpropertiesweretestedbylaserparticlesizeanalyzer,scanningelectronmicroscopy(SEM)andX-raydiffractometer(XRD).Thetestresultsshowedthatifthefiberslurrymovedfrom20to40circulationsthroughthehomogenizer,for10%ofthemicro/nanofibrils,theparticlesizewasreducedfrom1.2μmto160nmanditssurfaceareaperunitvolumewasincreasedfrom1.63×104...
简介:WecollectedacornsfromselectedQuercusroburandQuercuspetraeamothertreesgrowingatstationswithdifferentdepthstothewatertable.Weproducedpairsofmicro-cuttingsfromthesesingleacorns.Theobtainedpairsofcuttingsrepresentedphenotypesofhighsimilarityaccordingtophotosyntheticparameters.Thesepairsofcuttingswereusedtotesttheplasticityofresponsetocontrastingwaterregimes.Foreachpair,onecuttingwassubmittedtodroughtandtheotherwassubmittedtowaterlogging.Droughtresponsewasrecordedaccordingtogasexchangetraits(stomatalclosureandwateruseefficiency).TheresponsetowaterloggingwasrecordedaccordingtothecapacitytoformhypertrophiedlenticelsandtomaintaintheCO2assimilationrate.Intermediaryphenotypespresentingbothhighwateruseefficiencyandthecapacitytodevelophypertrophiedlenticelswereexpected.