简介:HuGuangLampFactoryinShanghaihasdevelopedelectricity-savinglampsincollaborationwithFudanUniversity,usingRE-containingthreeprimaryphos-phors.Thefactoryproducesseveralvarietiesoflamps,includingH-shaped,hyperbolicandDouble-Dlampsofdifferentwattagese.g.9,11,13,16and28w.A16wDouble-Dlampisasbrightasa85wordinaryin-candescencebulbandthelifeisthree
简介:摘要目的利用逆转录-环介导等温扩增技术(reverse transcription loop-mediated isothermal amplification,RT-LAMP)建立快速检测委内瑞拉马脑炎病毒(Venezuelan equine encephalitis virus, VEEV)的方法。方法针对VEEV nsp1基因序列,设计了5套特异性引物进行筛选,再对反应温度进行优化,最后进行特异性、灵敏性及重复性评价。结果RT-LAMP检测VEEV的最适温度在65 ℃;同时选取11种病毒进行RT-LAMP,证明了RT-LAMP具有高特异性;运用RT-qPCR与RT-LAMP对比灵敏性,结果表现为RT-LAMP检测的灵敏性超RT-qPCR检测的100倍。针对VEEV进行RT-LAMP的重复性实验,结果为具有较好的重复性。结论RT-LAMP检测VEEV法具有简单快速高效的特点,且装置简单,特异性强、灵敏性高、重复性好。
简介:Anegativeunipolarpulsedvoltageisappliedtostudyinternalelectricalparametersofthexenonfilleddielectricbarrierdischarge(DBD)sources.TheVUVradiationemittedfromthesesourcesischaracterizedbymeansofthephotoluminescenceintensityoftheredphosphorpellet.TheredphosphorconvertstheVUVradiationintovisibleradiationandtheemissionspectraincludeapeakat619.56nm.Theemissioncharacteristicsoftheredphosphorareanalyzedintermsofthepressure-distance(pd),risetimeandfrequencyofthepulsedvoltagewaveform.Theemissionintensitymeasuredatdifferentoperationalconditionsconfirmsthattheformationanddecayofthexenonexcimer,Xe2*,increasewiththeincreaseinreducedelectricfield,E/N.AfterexceedingcertainlimitsofE/N,theintensityofXe2*decreasesrapidly.
简介:AccordingtothenoticeoftheOfficeofPlanned,SafeandEffectiveUseofElectricity(shortasOPSEUE),allnon-industrialconsumers,suchashotels,shoppingcenters,areaskedtouseenergysavinglampsinsteadofincandescentlampandtoeliminateinductiveballastswithinthecoming3years.
简介:Understandingtheradiationmodelofaflashlampisessentialforthereflectordesignofalaseramplifier.Reflectordesignofteninvolvesseveralsimplifyingassumptions,likeapointorLambertiansource;eitheroftheseassumptionsmayleadtosignificanterrorsintheoutputdistribution.Inpractice,sourcenon-idealitiesusuallyresultinsacrificingtheamplifier’sgaincoefficient.Weproposeanoveltesttechniqueforattainingthexenonflashlampabsolutespectralintensityatvariousanglesofview,andthenaccuratelypredictradiationdistributionsandgeneratethereflectorshape.Itisshownthatduetotheabsorptionofemittedradiationbythelampitself,thebehavioroftheradiationmodelatvariouswavelengthsisdifferent.Numericalresultsofxenonplasmaabsorptioncoefficientwerecomparedwiththemeasureddata.Areasonableagreementwasobtainedfortheabsorptioncoefficientparameters.Thus,thisworkprovidesausefulanalyticaltoolfortheengineeringdesignoflaseramplifierreflectorsusingxenonflashlampsaspumps.
简介:摘要本文主要介绍LAMP,WordPress等现今流行的web技术架构,并通过实例详细介绍如何搭建基于上述技术架构的个人网站。在搭建完毕后,本文继续介绍网站的管理方式,最后阐述基于LAMP和WordPress的个人网站的应用价值及前景。
简介:目的:探索LAMP2分子对肝癌细胞增殖的影响及其机制。方法:首先利用Westernblot及QT-PCR技术检测在不同肝癌细胞系中LAMP2分子的表达水平,然后选择一种肝癌细胞系HepG2进行慢病毒转染以建立LAMP2沉默的稳定细胞系HepG2-9455。利用MTT法对比LAMP2沉默细胞系和未沉默细胞系细胞的增殖情况。检测其可能影响的几个关键信号通路分子如PI3K、AKT、NF-κB、ERK等以明确其作用机制。结果:LAMP2在各肝癌细胞系中普遍表达较高,其在正常肝组织中主要分布于胆管周围并且呈极性分布,然而在肝癌组织中LAMP2的定位却发生了紊乱。通过慢病毒构建的稳定细胞系HepG2-9455其LAMP2表达下降了90%,而在下调LAMP2后可以显著抑制肝癌细胞HepG2的增殖。并且在下调LAMP2分子后HepG2细胞的PI3K、AKT和NF-κB分子发生明显下调。结论:LAMP2分子可以通过调节下游的PI3K、NF-κB(pp65)分子而影响肿瘤细胞的增殖。提示LAMP2分子在肝癌的恶性进展中可能发挥了重要的作用。
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