简介：目的：探究人参内生细菌gel5菌株对人参病原菌的作用机理，明确ge15菌株胞外分泌液中抑菌活性成分的提取方法、活性组分及抑菌活性物质的稳定性。方法：通过硫酸铵沉淀、浓盐酸沉淀及饱及正丁醇萃取等方法获得菌株发酵液中抑菌活性成分；牛津杯法测定提取成分的抑菌活性。结果：从ge15菌株发酵液得到的提取液均有抑菌活性；对发酵上清液经高温和蛋白酶K处理，抑菌活性未见明显变化。结论：ge15菌株能产生多种抑菌活性成分；抑菌成分对高温和蛋白酶K稳定。

简介：Theuterinetetaniccontractionanduterinearterybloodflowreductionarepossiblereasonsforprimarydysmenorrhea（PD）.Inthepresentstudy,weaimedtoevaluatetheuterinerelaxanteffectandtheinfluenceonuterinearterybloodvelocityofGe-GenDecoction（GGD）,awell-knownChineseherbalformula.InfemaleICRmice,uterinecontractionwasinducedbyoxytocinexposurefollowingestradiolbenzoatepretreatment,andtheuterinearterybloodvelocitywasdetectedbyDopplerultrasound.HistopathologicalexaminationoftheuterinetissuesampleswereperformedbyH&Estaining.Exvivostudiesdemonstratedthatoxytocin,posteriorpituitary,oracetylcholineinducedcontractionsinisolatedmouseuterus.GGDinhibitedbothspontaneousandstimulatedcontractions.InvivostudydemonstratedthatGGDsignificantlyreducedoxytocin-inducedwrithingresponseswithamaximalinhibitionof87%.FurtherstudydemonstratedthatGGDnormalizedoxytocin-inducedabnormalitiesofprostaglandinsF2alpha(PGF2α)andCa2+inmice.Inaddition,injectionofoxytocininducedadecreaseinuterinearterybloodflowvelocity.PretreatmentwithGGDreversedtheoxytocinresponseonbloodflowvelocity.HistopathologicalexaminationshowedpretreatmentwithGGDalleviatedinflammationandedemaintheuteruswhencomparedwiththemodelgroup.BothexvivoandinvivoresultsindicatedthatGGDpossessedasignificantspasmolyticeffectonuterinetetaniccontractionaswellasimprovementonuterinearterybloodvelocitywhichmayinvolvePGF2αandCa2+signaling,suggestingthatGGDmayhaveaclinicpotentialinPDtherapy.

简介：根据中药方剂研究特点,按正交设计原理及F值与t值的特定关系,提出“正交t值法”。数理上与正交设计F值法等价,原理清晰,计算简便,统计结论完全一致。实验分三步进行(主药分析,辅药交互分析,剂量选择),并改进了正交表形式,便于分析两药间的协同或拮抗作用,适用于中药较大方剂的研究。主要公式为:残差标准误自由度显著性检验二水平时预期药效二水平时