The Mutation Breeding and Mutagenic Effect of Air Plasma on Penicillium Chrysogenum-中国期刊网

摘要 Lowtemperatureairplasmawasusedasthemutationtoolforpenicillin-producingstrainPenicilliumchrysogenum.ThedischargeconditionswereRFpowerof360W,temperatureof40℃inasealedchamber,andpressureof10Pato30Pa.Theresultshowedthatthekineticsofthesurvivalratefollowedatypicalsaddle-shapedcurve.Basedonastatisticanalysis,atthetreatingdurationof10min,thepositivemutationratewasashighas37.5%whilethenegativemutationratewaslow.Thecolonialmorphologychangedobviouslywhentheplasmatreatingdurationreachedorexceeded45min.Afterbothprimaryandsecondaryscreening,amutantdesignatedasaPc051310withhighproductivityofpenicillinwasobtained,andastrongmutageniceffectonP.chrysogenumwasobservedintheprocess.Itwasprovedthatafterfivegenerations,themutantaPc051310stillexhibitsahighproductivity.Alltheresultsprovethattheplasmamutationmethodcouldbedevelopedasaconvenientandeffectivetooltobreedhigh-yieldstrainsinthefermentationindustry,whileexpandingtheplasmapplicationatthesametime.

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The Mutation Breeding and Mutagenic Effect of Air Plasma on Penicillium Chrysogenum

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The Mutation Breeding and Mutagenic Effect of Air Plasma on Penicillium Chrysogenum

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