Effects of HLEC on the secreted proteins of epithelial ovarian cancer cells prone to metastasize to lymph nodes-中国期刊网

摘要 Objective:TostudyexplorestheeffectofHLEConthesecretedproteinsofepithelialovariancancer(EOC)cells(SKOV3-PM4)withdirectionalhighlylymphaticmetastasis.Methods:Supernatantsoffourgroupsofculturedcells,namely,SKOV3(A),SKOV3+HLEC(B),SKOV3-PM4(C),SKOV3-PM4+HLEC(D),werecollected,andtheirproteinsweredetectedbyantibodyarraysandiTRAQ-2D-LC-MALDITOF/TOF/MS.SignificantlydifferentialproteinswerefurtheranalyzedviabioinformaticsandvalidatedinhumanserumsandcellmediaviaELISA.Results:ResultsofantibodyarraysandmassspectrometrydemonstratedthatGRNandVEGFAwereupregulatedingroupC(comparedwithgroupA),whereasIGFBP7andSPARCweredownregulatedingroupD(comparedwithgroupC).ComprehensivebioinformaticsanalysisresultsshowedthatIGFBP7andVEGFAwerecloselylinkedtoeachother.FurthervalidationwithserumsshowedstatisticalsignificanceinVEGFAandIGFBP7levelsamonggroupsofpatientswithovariancancers,benigntumors,andcontrolgroups.Twoproteinswereupegulatedinthefirstgroup.VEGFAinthecontrolgroupwasdownregulated.ForIGFBP,upregulationinthecontrolgroupanddown-regulationinthefirstgroupwerealsoobserved.Conclusion:TheHLECmicroenvironmentiscloselyassociatedwithdirectionalmetastasistolymphnodesandwithdifferentialproteinsincludingcellstromalproteinsandadhesionfactors.TheupregulationofVEGFAandGRNandthedownregulationofSPARCandIGFBP7arecloselyassociatedwithdirectionalmetastasistolymphnodesinEOCcells.

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Effects of HLEC on the secreted proteins of epithelial ovarian cancer cells prone to metastasize to lymph nodes

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Effects of HLEC on the secreted proteins of epithelial ovarian cancer cells prone to metastasize to lymph nodes

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