摘要
Inthisstudy,weexaminedtheeffectofelevatedtemperatureontheexpressionpatternsofgenes,i.e.,nacrein,irr,n16,n19,andhsp70inthepearloysterPinctadafucata.Theexperimentwascarriedoutat4temperatures,i.e.,20℃(ambient,control),24,28℃,and32℃.TheexpressionlevelsoftargetgenesinP.fucatawereassayedat0,6,24,48,and96hviareal-timepolymerasechainreaction.Resultsshowedthattheexpressionlevelsofnacreinandirrhadnosignificantvariationsamongdifferenttimepointsbelow28℃,butsignificantlyincreasedovertimeat32℃.Theexpressionlevelsofn16andn19didnotchangemarkedlyat20℃.Theformerincreasedsignificantlyat6hand24hwhilethelattersubstantiallydecreasedduring6–96hat24,28and32℃.Amongdifferenttemperatures,thelevelofn16wassignificantlylowerat20℃thanatothertemperaturesduring6–96h,andthelevelofn19significantlyvariedamongdifferenttemperaturesat48hand96h.Theexpressionlevelofhsp70wassignificantlyhigherat32℃thanat20,24and28℃at24h.TheseresultsdemonstratedthatelevatedtemperatureimpactedthephysiologicalprocessesofP.fucataandpotentiallyinfluenceditsadaptabilitytothermalstress.
出版日期
2014年03月13日（中国期刊网平台首次上网日期，不代表论文的发表时间）
