摘要
Theobjectiveofthestudywastoelucidateopticalcharacteristicsofthechromophorestructuresoffluorescentproteins.RamanspectraofcommonlyusedGFP-likefluorescentproteins(FPs)withdiverseemissionwavelengths(green,yellow,cyanandred),includingtheenhancedhomogenousFPsEGFP,EYFP,andECFP(fromjellyfish)aswellasmNeptune(fromseaanemone)weremeasured.High-qualityRamanspectrawereobtainedandmanymarkerbandsforthechromophoreoftheFPswereidentifiedviaassignmentofRamanspectrabands.WereportthepresenceofapositivelinearcorrelationbetweentheRamanbandshiftofCs=C6andtheexcitationenergyofFPs,demonstratedbyplottingabsorptionmaxima(cm-1)againstthepositionoftheRamanbandCs=C6inEGFP,ECFP,EYFP,theanionicchromophoreandtheneutralchromophore.ThisstudyrevealednewRamanfeaturesinthechromophoresoftheobservedFPs,andmaycontributetoadeeperunderstandingoftheopticalpropertiesofFPs.
出版日期
2018年05月15日(中国期刊网平台首次上网日期,不代表论文的发表时间)