摘要
Brassinosteroid(BR)andgibberellin(GA)aretwopredominantplanthormonesthatregulateplantcellelongation.Mutantsdisruptthebiosynthesisofthesehormonesanddisplaydifferentdegreesofdwarfphenotypesinrice.Althoughtheroleofeachplanthormoneinpromotingthelongitudinalgrowthofplantshasbeenextensivelystudiedusinggeneticmethods,theirrelationshipisstillpoorlyunderstood.Inthisstudy,weusedtwospecificinhibitorstargetingBRandGAbiosynthesistoinvestigatetherolesofBRandGAingrowthofriceseedlings.Yucaizol,aspecificinhibitorofBRbiosynthesis,andTrinexapac-ethyl,acommerciallyavailableinhibitorofGAbiosynthesis,wereused.TheeffectofYucaizolonriceseedlingsindicatedthatYucaizolsignificantlyretardedstemelongation.TheIC50valuewasfoundtobeapproximately0.8μmol/L.Yucaizolalsoinducedsmallleafanglephenocopyinriceseedlings,similarlytoBR-deficientrice,whileTrinexapac-ethyldidnot.WhenYucaizolcombinedwithTrinexapac-ethylwasappliedtothericeplants,themixtureofthesetwoinhibitorsretardedstemelongationofriceatlowerdoses.OurresultssuggestthattheuseofaBRbiosynthesisinhibitorcombinedwithaGAbiosynthesisinhibitormaybeusefulinthedevelopmentofnewtechnologiesforcontrollingriceplantheight.
出版日期
2016年01月11日(中国期刊网平台首次上网日期,不代表论文的发表时间)