摘要
Microbecommunitiesinrhizosphereecosystemsareimportantforplanthealthbutthereislimitedknowledgeofthemintherhizospheresofgeneticallymodified(GM)plants,especialfortreespecies.WeusedtheamplitudesequencingmethodtoanalyzetheV4regionsofthe16SrRNAgenetoidentifychangesinbacterialdiversityandcommunitystructureintwoGMlines(D520andD521),onenon-geneticallymodified(nonGM)lineandinuncultivatedsoil.Afterchimerafiltering,468.133sequencesinthedomainBacteriaremained.Thereweretendominanttaxonomicgroups(with[1%ofallsequences)acrossthesamples.241of551genera(representingaratioof97.33%)werecommontoallsamples.AVenndiagramshowedthat1.926operationaltaxonomicunits(OTUs)weresharedbyallsamples.Wefoundaspecificchange,areductioninChloroflexi,inthemicroorganismsintherhizospheresoilplantedwithpoplars.Takentogether,theresultsshowedfewstatisticaldifferencesinthebacterialdiversityandcommunitystructurebetweentheGMlineandnon-GMline,thissuggeststhattherewasnoorverylimitedimpactofthisgeneticmodificationonthebacterialcommunitiesintherhizosphere.
出版日期
2016年04月14日（中国期刊网平台首次上网日期，不代表论文的发表时间）
