摘要
Thecharacteristicsoftheparticulatemousecentromereenrichedfractionfromisolatednucleiobtainedinourlaboratorywereinvestigatedbyindirectimmunofluorescence,testoftheactivityofmicrotubuleorganizingcenter(MTOC),SDS-PAGE,andfluorescenceinsituhybridization.MostoftheparticlesofthefractionarecomplexesofDNAandkinetochoreproteinsandshowMTOCactivity.TheDNAisolatedfromthefractioncanhybridizewithDNAintheregionsoftheprimaryconstrictionsofallchromosomesofascionsoftheprimaryconstrictionsofallchromosomesofascitescells.Thekinetochoreproteinsisolatedfromthefractionaremainlythosewithmolecularweightof55KDand59KD.Resultssuggestedthatthefractionobtainedisacentromereenrichednuclearfractionasindicatedinourpreviousreport.
出版日期
1993年02月12日(中国期刊网平台首次上网日期,不代表论文的发表时间)