摘要
ExpressedSequenceTag(EST)analysishaspioneeredgenome-widegenediscoveryandexpressionprofiling.Inordertoestablishageneexpressionindexinthericecultivarindica,wesequencedandanalyzed86,136ESTsfromninericecDNAlibrariesfromthesuperhybridcultivarLYP9anditsparentalcultivars.WeassembledtheseESTsinto13,232contigsandleave8,976singletons.Overall,7,497sequenceswerefoundsimilartotheexistingsequencesinGenBankand14,711arenovel.Thesesequencesareclassifiedbymolecularfunction,biologicalprocessandpathwaysaccordingtotheGeneOntology.WecomparedoursequencedESTswiththepubliclyavailable95,000ESTsfromjaponica,andfoundlittlesequencevariation,despitethelargedifferencebetweengenomesequences,Wethenassembledthecombined173,000riceESTsforfurtheranalysis.UsingthepooledESTs,wecomparedgeneexpressioninmetabolismpathwaybetweenriceandArabidopsisaccordingtoKEGG.Wefurtherprofiledgeneexpressionpatternsindifferenttissues,developmentalstages,andinaconditionalsterilemutant,aftercheckingthelibrariesarecomparablebymeansofsequencecoverage.Wealsoidentifiedsomepossiblelibraryspecificgenesandanumberofenzymesandtranscriptionfactorsthatcontributetoricedevelopment.
出版日期
2003年01月11日(中国期刊网平台首次上网日期,不代表论文的发表时间)