摘要
Assessmentofgeneticdiversityisanessentialcomponentingermplasmcharacterizationandconservation.TherearethreewildricespeciesinHainanProvince,includingOryzarufipogonGriff.InordertodetectthegeneticdiversityofdifferentpopulationsofOryzarufipogoninHainan,ISSR(inter-simplesequencerepeat)andSSR(simplesequencerepeat)markerswereusedtoinvestigate180accessionsfromsixlocalitiesinHainan.FourteenISSRprimersamplified185alleleswith171(92.43%)polymorphic,thenumberofallelesrangedfrom8to17,withanaverageof13.14allelesperlocus.Thirty-eightpairsofSSRprimersusedinthisstudyamplified213alleleswith190(89.20%)polymorphic,thenumberofallelesrangedfrom2to14,withanaverageof5.66allelesperlocus.BothISSRandSSRanalysesrevealedahighlevelofgeneticdiversityinthewildpopulations.ThepopulationwiththehighestgeneticdiversityisWanning(WN),andthepopulationwithlowestgeneticdiversityisWenchang(WC).TheresultsofaUPGMAclusterusingtheNTSYSprogramshowedthateachpopulationhasalowdegreeofgeneticdifferentiation.Furthermore,theManteltestrevealedthatthegeneticsimilaritiesdetectedbyISSRandSSRweresignificantlycorrelated(r=0.8634,t=93.67)whendetectinggeneticdiversityatthespecieslevel.ThetwomolecularmarkersystemswereabletodeterminethegeneticdiversityamongOryzarufipogon,andthetwogroupsofindexesobtainedbyusingthetwomarkershaveahighlevelofconsistency.
出版日期
2018年01月11日（中国期刊网平台首次上网日期，不代表论文的发表时间）
