摘要
Objective:Toinvestigatetheculturemethodofskin-derivedprecursors(SKPs)andtoexploreanewcellsourceforcelltransplantationofcentralnervoussystem.Methods:Cellsfromskinsofjuvenileandadultmicewereisolatedandculturedinserum-freemedium.Amechanicalmethodwaschosentopassagethesecellsandtheywereidemifiedbytheimmunocytochemistryassay.Results:SKPscouldbeisolatedfromadultandneonatalskins.Theycouldbemaintainedinvitroforlongperiodswithstableproliferation,andexpandedasundifferentiatedcellsincultureformorethan12passages.About50％ofSKPsexpressednestinandmajorityofthesecellsexpressedfibronectinwhentheywereplatedonpolyornithineandlaminincoatedplates.About5％cellsshowedneuronaldifferentiationandexpressedneurofilament-M(NF-M)andNSEwhenSKPswereplatedinserun-containingmedium,andthesecellscouldalsodifferentiateintoadipocytesandfibroblast-likecells.Conclusions:Thedatasupportthehypothesisthatadultskincontainsstemcellscapableofdifferentiatingintoneurons,adipocytes,andfibroblast-likecells.TheymayrepresentanalternativeautologousstemcellsourceforCNScelltransplantation.
出版日期
2004年02月12日（中国期刊网平台首次上网日期，不代表论文的发表时间）
