Reversal of 5-flouroucial resistance by adenovirus-mediated transfer of wild-type p53 gene in multidrug-resiatant human colon carcinoma LoVo／5-FU cells-中国期刊网

摘要 AIM:Toobservethereversaleffectsofwide-typep53geneonmulti-drugresistanceto5-FU(LOVO/5-FU).METHODS:AftertreatmentwithAd-p53,LOVO/5-FUsensitivityto5-Fuwasinvestigatedusingtetrazoliumdyeassay.Multidrugresistancegene-1(MDR1)geneexpressionwasassayedbysemi-quantitativereversetranscriptionpolymerasechainreactionandtheexpressionofp53proteinwasexaminedbyWesternblotting.RESULTS:Thereversalactivityaftertreatmentwithwidetypep53genewasincreasedupto4.982foldat48h.TheexpressionofMDR1genedecreasedsignificantlyaftertreatmentwithwide-typep53gene,andtheexpressionofp53proteinlastedforabout5d,withapeakat48h,andbegantodecreaseat72h.CONCLUSION:Wide-typep53genehasaremarkablereversalactivityforthehighexpressionofMDR1geneincolorectalcancers.Thereversaleffectsseemtobeinatimedependentmanner.Itmighthavegoodprospectsinclinicalapplication.

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Reversal of 5-flouroucial resistance by adenovirus-mediated transfer of wild-type p53 gene in multidrug-resiatant human colon carcinoma LoVo／5-FU cells

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Reversal of 5-flouroucial resistance by adenovirus-mediated transfer of wild-type p53 gene in multidrug-resiatant human colon carcinoma LoVo／5-FU cells

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