摘要
Toinvestigatetheglutamate-likeimmunoreactivity(Glu-IR)changesininnerhaircells(IHCs)ofguineapigscochlearafternoiseexposure.Guineapigsweredistributedinto6groupsincludingcontrolgroup(gl),andgroupsofimmediately(g2),8hours(g3),1day(g4),3days(g5)and7days(g6)afternoiseexposure.Theexperimentalgroupswereexposedto120dBLp1/3octave4kHznarrowbandnoisefor4hourstoruintheorganofCorti.ThefirstturnoftheorganofCortiwasdissectedtomakeultrathinsectionsandunderwentimmunoelectronstudy.ThedensityofgoldparticlesofGlu-IRinIHCswasmeasured.ResultsshowedthatGlu-IRinIHCsafternoiseexposurechangeddynamically.ThedensityofgoldparticleinIHCsofg2significantlyincreased(p<0.001)comparedwithglanddecreaseding3(p<0.001),whereastherewerenosignificantdifferenceinthegroupsofg4,g5andg6(p>0.05).TheresultssuggestthattheremaybeglutamateautoreceptorsinthemembraneofIHCsandtheglutamatereleasedfrompre-synapsemayactontheseautoreceptorsinapostitivefeedbackmanner,whichinduced[Ca^2+]increasedinIHCsimmediatelyafternoiseexposure.GlutamatesynthetasesystemwasactivatedbyCa^2+andmoreglutamicacidwasproduced.Inthegroupof8hoursafternoiseexposure,glutamicaciddecreasedinIHCsbecauseofitsover-releasingintosynapses.Glu-IR'srecoverytonormallevelindicatedthattheglutamateglutaminecyclemayexistinguineapigcochlea.
出版日期
2004年04月14日（中国期刊网平台首次上网日期，不代表论文的发表时间）
