Resolution improvement of electron micrographs by the direct method for biotin-binding protein streptavidin-中国期刊网

摘要 Direct-methodphaseextensionhasbeenappliedtotwo-dimensionalelectrondiffractiondataoftheproteinstreptavidin.Structure-factoramplitudesfromelectrondiffractionwerecombinedwithphasesfromthecorrespondingelectronmicrographs.Maximum-entropydiscriminationandclusteranalysiswereusedtoderiveasolutionfromalargenumberofrandomtrials.Thephaseextensionfrom0.3to0.25nmledtosubstantialimprovementofthereconstructedprojectionimagequality.

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Resolution improvement of electron micrographs by the direct method for biotin-binding protein streptavidin

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Resolution improvement of electron micrographs by the direct method for biotin-binding protein streptavidin

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