摘要
Aim:TocomparetherecoveryrateofmorphologicallynormalandchromatincondensedspermatozoafromnativesemensamplesusingtheSpermPrepTMfiltrationcolumnsandPercollgradientcentrifugationandtodeterminetheinfluenceofthetwoprocessingtechniquesonfertilizationandpregnancyratesinanIVF-ETprogram.Methods:Sixteensemensamplesobtainedfrompatient'shusbandwereincludedinthisstudy.Eachwasdividedintotwoaliquots.ThefirstaliquotwasprocessedwithSpermPrepTMfiltrationcolumnsandthesecond,Percollgradientcentrifugation.Smearsweremadebeforeandaftersemenprocessingwithbothmethodsfortheevaluationofchromatincondensation(chromomycineCMA3)aswellasmorphology(strictcriteria)ofspermatozoa.Onehundredandseventyoocyteswereretrievedfromthepatientsandtheoocytesfromeachpatientweresubdividedintotwosets:onesetwasinseminatedusingspermatozoaprocessedwithSpermPrepTMandtheotherinseminatedaftersemenprocessingwithPercollgradientcentrifugation.Resuits:ThePercollmethodyieldedasignificantlyhigherpercentageofchromatincondensed(90.8±6.5%vs82.3±8.8%,P=0.017)andmorphologicallynormalspermatozoa(12.9±7.4%vs6.9±4.8%,P=0.001)incomparisontoSpermPrepTM.Whereas,spermcountrecoveryratewassignificantlyhigheraftertheuseofSpermPrepTMthanafterthePercollgradientcentrifugation.Thefertilizationratewassimilarbetweenthetwomethods.Conclusion:SemenprocessingwithPercollshouldberecommendedforintracytoplasmicsperminjectionasthenaturalselectionisbypassedandtheSpermPrepTMtechniquecouldberecommendedfor1VFandIUIprogramsasthespermconcentrationplaysamoresignificantroleintheseprocedures.
出版日期
2001年02月12日（中国期刊网平台首次上网日期，不代表论文的发表时间）
