Establishment of animal model for potency evaluation of inactivated SARS virus experimental vaccine-中国期刊网

摘要 ThepurposeofthisstudywastotesttheeffectivenessofsourcevirusstrainforthemanufactureoftheinactivatedSARSvirusvaccine,andestablishanexperimentalmethodandpreliminarystandardforpotencyevaluation.MiceweredividedintogroupsforbeingimmunizedwithcorrespondingseriallydilutedexperimentalSARSvirusinactivatedvaccine.Andtherabbitswereimmunizedwithundilutedvaccine.ChallengeassaywasconductedwithaheterologousSARSvirus.Andtheneutralizationantibodywasdeterminedwithplaquereductionneutralizationtest(PRNT),towhichtheneutralizationantibodyintheconvalescentserumofSARSpatientswascompared.Theexperimentalvaccineviralstrainswereprovedtobesuitableformanufacturingthevaccine.Miceimmunizedbyvaccinesofserialdilutionswereabletoelicitneutralizingantibody.Theantibodytiterfrommiceimmunizedwiththeundilutedvaccinecouldreachupto1:495.2,whilethoseofrabbitsimmunizedwiththeundilutedvaccinecouldreachaGMTof55.0-79.9.ThecapabilityoftheantibodytoneutralizethevirusfromGuangdongismoreefficientthanthatfromBeijing.TheGMTofneutralizingantibodyinSARSconvalescentslivinginsouthandnorthChinarangedfrom50.12to54.95,andthetitersofconvalescentsfromnorthChinawerehigherthanthosefromsouthChina.Miceandrabbitsusedasthemodelforevaluationofpotencyareofsensitivity,andthetestisofreproducibility.Thecandidatechallengeviralstrainsshowedarelativelyconsistenteffectonevaluatingantibodiesproducedbyvariousbatchesanddifferentvaccine-sourcestrains,hencetheycanbeusedtoevaluatepotencyofthevaccine.Themethodfortestingthevaccinepotencyandtheevaluationstandardwasestablishedpreliminarily.

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Establishment of animal model for potency evaluation of inactivated SARS virus experimental vaccine

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Establishment of animal model for potency evaluation of inactivated SARS virus experimental vaccine

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