Isolation and Characterization of Exosomes Derived from Tumor Cells Genetically Expressing Model Antigen-中国期刊网

摘要 Tumorcell-derivedexosomeshavebeenproposedasnon-cellularnanomericvaccinewhichcouldinducepotentantitumorimmuneresponseinmice.Inordertodeveloptheprotocolstopreparetumorcell-derivedexosomesforbasicresearchandclinicaltrail,weisolatedexosomesfromovalbumin(OVA)-expressingthymomacellsEG.7-OVAbyvariouspreparationmethods.Wedemonstratethenon-sedimentationmethodissimple,rapid,efficientwithhigheryieldandpurityofexosomes.EG.7-OVA-derivedexosomesare40-100nmindiametersequesteredbylipidbi-layer,andcontainrichheatshockprotein(HSP)andOVA.Theresultofthesizedistributiondeterminationisconsistentwiththecalculationbythevisualmicroscopicinspection,with90.4%particlesattherangeof50-90nm.Moreover,asamodelantigenoftheEG.7cells,OVAconcentrationinEG.7-derivedexosomescanberegardedasagoodqualitycontrolparameter.Therefore,wehaveestablishedaplatformtoefficientlyprepareexosomesfortumorimmunotherapy.

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Isolation and Characterization of Exosomes Derived from Tumor Cells Genetically Expressing Model Antigen

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Isolation and Characterization of Exosomes Derived from Tumor Cells Genetically Expressing Model Antigen

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