Steady state and time-resolved autofluorescence studies of human colonic tissues-中国期刊网

摘要 Steadystateandtime-resolvedautofluorescencespectroscopiesareemployedtostudytheautofluorescencecharacteristicsofhumancolonictissuesinvitro.Theexcitationwavelengthvariesfrom260to540nm,andthecorrespondingfluorescenceemissionspectraareacquiredfrom280to800nm.Significantdifferenceinfluorescenceintensityofexcitation-emissionmatrices(EEMs)isobservedbetweennormalandtumorcolonictissues.Comparedwithnormalcolonictissue,lownicotinamideadeninedinucleotide(phosphate)(NAD(P)H)andflavinadeninedinucleotide(FAD),andhighaminoacidsandprotoporphyrinIX(PpIX)fluorescencescharacterizehigh-grademalignanttissue.Moreover,theautofluorescencelifetimesofnormalandcarcinomatouscolonictissuesat635nmunder397-nmexcitationareabout4.32±0.12and18.45±0.05ns,respectively.ThehighaccumulationofendogenousPpIXincoloniccancersisdemonstratedinbothsteadystateandtime-resolvedautofluorescencespectroscopies.更多还原

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Steady state and time-resolved autofluorescence studies of human colonic tissues

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Steady state and time-resolved autofluorescence studies of human colonic tissues

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