摘要
Objective:TodetecttheeffectofarsenictrioxideorATRAonAPLcellsorHL-60cellsandtoinvestigatethemechanismofthehyperleukocytosisanddetectthecrossresistancebetweenATRAandarsenictrioxide.Methods:Thenumberofpromyelocytesormorematuredgranulocyteswerecountedbyregularmethod,MTTtestwasusedtomeasuretheproliferationofHL-60cellsorAPLcells,flowcytometryanalysistomeasuretheapoptosis,NBTmethodtodetectthedifferentiationofHL-60cellsorAPLcells.Results:TheproliferationofprimaryAPLcellsorHL-60cellscouldbeinhibitedinvitrobyeitherarsenictrioxideorATRA,whichcouldinduceobviousapoptosisorobviousdifferentiationofprimaryAPLcellsorHL-60cells.InhibitionofproliferationorapoptosisofATRAresistantHL-60cellswereachievedbyexposuretoarsenictrioxideinvitro.Ontheotherhand,theresultsofinvivotreatmentshowedthatarsenictrioxidealsoinduceofhyperleukocytosis.Conclusion:TheresultsindicatedthatthehyperleukocytosisinducedbyATRAisnotcontributedtothemechanismofmoredifferentiationthanapoptosis,therewasnotcrossresistancebetweenATRAandarsenictrioxide.
出版日期
2001年02月12日(中国期刊网平台首次上网日期,不代表论文的发表时间)