摘要
TheproteomicsofthedifferentialproteinexpressionsinhumangliomacelllineU251cellsinfectedwithhumancytomegalovirus(HCMV)wasinvestigatedattheproteinlevelbyusingthesurfaceenhancedlaserdesorption/ionization(SELDI)proteinchipsysteminordertodevelopamethodofstudyforthepathogenesisofHCMVinfection.Inthisstudy,theculturedU251cellswereinfectedwithHC-MVingoodconditionandthesupernatantsoflysatesandtheextracellularfluidsofthecultivatedinfect-edcellswerequantitativelydefinedfortheexpressedproteins.TheproteomicsofthedifferentialproteinexpressionincellsbeforeandafterinfectionwasanalyzedbyWCX2arraysontheproteinchipreader.Itwasdemonstratedthatthecytopathiceffectsofinfectedcellsappearedonthe5thdayafterinfection,however,thedifferentialproteinexpressionwasevidentat6hafterinfectionasrevealedbyRT-PCRandmassspectrometry.Theproteinpeakscapturedfromdifferentbatchesofsamples,fromthesamesampledetectedwithdifferentarraysorforthedifferentlimeswereallequivalent.Withthemolecularweightrangefrom2000Dato3000Da,chipcaptured82peaksfromtheintracellularfluidsand11proteinpeakfromthecellularfluidinwhichcomparedwiththecontrolgroup,theproteinpeakswithmolecularweightof13536.3Da,10046.1Daand17106.2Dawereclosetothoseofβ-amyloidpro-tein,caspase-1precursorandLPS-inducedTNF-αfactorrespectively,whichshowedbriefup-regulation4hafterinfection,andcontinuedtoraise48hlater.Theseresultsinferthattheseproteinsmaybere-latedtotheapoptosisinducedbyHCMVinfection,thussuggestingthattheapeptosisinducedbyHC-MVinfectionmayplayaroleinthepathogenesisofHCMVinfection.
出版日期
2007年01月11日(中国期刊网平台首次上网日期,不代表论文的发表时间)