摘要
AIM:Toinvestigatetheeffectsofthefreeradicalscavengerbis(1-hydroxy-2,2,6,6-tetramethyl-4-piperidinyl)decandioate(IAC)inthedextransodiumsulphate(DSS)experimentalmodelofulcerativecolitis.METHODS:ColitiswasinducedinSpragueDawleymaleratsbyadministrationof5%DSSindrinkingwater.IAC(30mg/kg,lipophilicorhydrophilicform)wasadministereddaily(orallyorip)for6duntilsacrifice.Colonicdamagewasassessedbymeansofindirect(DiseaseActivityIndexscore)anddirectmeasures(macroscopicandmicroscopicscores)andmyeloperoxidase(MPO)activity.NeutrophilinfiltrationwithinthetissueandglutathioneS-transferaseactivitywerealsoinvestigated.RESULTS:DSS-inducedcolitisimpairedbodyweightgainandmarkedlyincreasedallinflammatoryparameters.Six-daytreatmentwithlipophilicIACsignificantlyreducedintestinaldamagecausedbyinflammation,inducedadown-regulationinMPOactivity(0.72±0.12and0.45±0.12withlipophilicIACpoandip,respectively,vs1.10±0.27inuntreatedDSScolitisanimals)andminimizedDSS-inducedneutrophilinfiltration,whilehydrophilicIACadministeredorallydidnotameliorateDSS-induceddamage.CONCLUSION:TheseresultssupportthehypothesisthatreactiveoxygenmetabolitescontributetoinflammationandthattheradicalscavengerIAChastherapeuticpotentialininflammatoryboweldisease.
出版日期
2010年12月22日（中国期刊网平台首次上网日期，不代表论文的发表时间）
