Micro-Raman spectroscopy of single leukemic cells

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摘要 TheRamanspectrafromleukemiccellline(HL60)andnormalhumanperipheralbloodmononuclearceils(PBMCs)areobtainedbyconfocalmicro-Ramanspectroscopyusingnear-infraredlaser(785nm)excitation.Thescanningrangeisfrom500to2000cm-1.ThetwoaverageRamanspectraofnormalPBMCsandcarcinomacellshavecleardifferencesbecausetheirstructureandamountofnucleicacid,protein,andothermajormoleculesarechanged.Thespectraarealsocomparedandanalyzedbyprincipalcomponentanalysis(PCA)todemonstratethetwodistinctclustersofnormalandtransformedcells.Thesensitivityofthistechniqueforidentifyingtransformedcellsis100%.更多还原
机构地区 不详
出版日期 2008年12月22日(中国期刊网平台首次上网日期,不代表论文的发表时间)
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