摘要
Asequentialstatisticalapproachwasappliedtooptimizingthefermentationmediumofepothilones(Epos)productionbymeansofamutantwhichwasobtainedbytreatingpolyangiumcellulosumATCC15384withnitriteandultraviolet.Theeffectsofdifferentcarbonsourcesandnitrogensourcesonthefermentationmediumweretested,andthesuitableoneswereselected.Thenauniformdesignwasemployedtodesigntheexperiments.Alinearmodelwasdevelopedforidentifyingthesignificantcomponentsinfermentationmedium,whileathirddegreepolynomialmodelwasusedforstudyingtherelationshipbetweentheconcentrationofthecomponentsinfermentationmediumandtheyieldofEpos(YEPs).Apatternsearchmethodwasusedforsearchingtheoptimumfermentationmediuminthetestspace,whichwasasfollows(g/L):potassiumnitrate8.00,soybeanpeptone17.60,potassiumhydrogenphosphate1.00,beefextraction6.46,yeastextraction1.00,calciumchloride0.25,sodiumchloride1.00andferricchloride0.02.TheoptimumfermentationmediumwasexpectedtoresultinayieldofEpos(YEPs)of2.48mg/L.ThevalidationexperimentswiththeoptimummediumwereperformedintriplicateandtheaverageyieldofEposwas2.45mg/Lwhichwas7.78timeshigherthanthatofEpospreparedwithoutoptimization.
出版日期
2010年01月11日（中国期刊网平台首次上网日期，不代表论文的发表时间）
