摘要
BACKGROUND:Livedeliverylimitstheclinicalapplicationofmaggottherapy.TodateinChina,therearenoinvivoreportsregardingwoundhealingmechanismsofmaggottherapyortheeffectsofmaggothomogenateonwoundnerveregeneration.OBJECTIVE:Toavoidcomplicationsduetotheuseoflivemaggots,anasepticmaggothomogenatewasapplied.SubstanceP(SP)andgeneproteinproduct9.5expressioninacutaneouswoundwasanalyzedtoexplorepossiblemechanismsofneuralregenerationandwoundhealingintherat.DESIGN,TIMEANDSETTING:ArandomgroupingandcontrolledanimalstudywasperformedatthelaboratoryoftheDepartmentofOrthopedicSurgery,FirstAffiliatedHospital,DalianMedicalUniversityfromAugust2008toApril2009.MATERIALS:LivemaggotswereculturedandprovidedbythelaboratoryoftheDepartmentofOrthopedicSurgeryoftheFirstAffiliatedHospital,DalianMedicalUniversity,China.METHODS:Atotalof48adultratswereselectedandtwoacute,full-thicknesswounds(round,1.5cmdiameter)werecreatedonthebackofeachrat.Thetwowoundswererandomlyassignedtohomogenateproductandcontrolgroups.Followingtwo-stepdisinfectionofmaggots,ahomogenatewasproducedfrom10maggotsandappliedtothewoundareainthehomogenateproductgroup,whilethewoundsinthecontrolgroupweretreatedwithnormalsalinealone.MAINOUTCOMEMEASURES:Ondays1,3,7,10,14,and21followinginjury,thewoundtissuewasexcised.HistologicalexaminationofthewoundwasobservedbyhematoxylinandeosinstainingorMasson'sTrichromestaining.SPandproteingeneproduct9.5expressionswereexaminedbyimmunohistochemistrytoevaluatewoundneuralregeneration.RESULTS:Ondays7,10,and14,therateofwoundhealingwassignificantlygreaterinthehomogenateproductgroupcomparedwiththecontrolgroup(P<0.05),andhomogenatehealingwasbetterthanthatseeninthecontrolgroup.Ondays3,7,and10,SPexpressionincellsandregenerativenerveswassignificantlygreaterinthehomogenateproductgroupcomparedw
出版日期
2010年08月18日（中国期刊网平台首次上网日期，不代表论文的发表时间）
