摘要
Thepurposeofthisstudyistoexploretheexpressionofgrowth-associatedprotein(GAP-43)inspinalcordsegmentsconnectedwithinjuredsciaticnervebythetreatmentwithbrazileininmice.Unilateralsciaticnerveinterruptionandanastomosiswereperformed.Physiologicalsaline(blankgroup),highdose,middledoseandlowdoseofbrazileinwereadministratedintragastricallytohealthyadultBALB/cmiceinseparategroups.L4―6spinalsegmentsconnectedwiththesciaticnervewereharvested.Real-timePCR(Polymerasechainreaction)andWesternblotanalysiswereperformedtodetecttheexpressionofGAP-43inspinalsegments.Histologicalstainingonmyelinandtheelectrophysiologywereperformedtoexaminethesciaticnerverecovery.GAP-43wasactivatedinspinalcordL4―6connectedwithinjuredsciaticnerve.Inthesurvivaltimeof12h,24h,3d,5d,7dand14d,GAP-43expressioninthemotorneuronsofspinalcordofthehighdosegroupandthatinthemiddledosegroupweresignificantlyhigherthanthoseonthelowdoseandblankgroups.MyelininthehighdosegroupandthatinthemiddledosegroupweremorematureandthepotentialamplitudeandMNCV(motornerveconductionvelocity)inthehighandmiddledosegroupswereobviouslyhigherthanthoseinthelowdosegroupandblankgroup.BrazileinfacilitatestheexpressionofGAP-43inneuronsinspinalcordL4―6segmentsconnectedwithinjuredsciaticnerve,whichpromotesnerveregeneration.
出版日期
2011年02月12日（中国期刊网平台首次上网日期，不代表论文的发表时间）
