简介：Clusterinisa75-80kDaheterodimericglycoprotein,thatisproducedinmosttissuesbutwhichexactbiologicalroleisstillnotclear.Particularly,itsroleinprotectionorpromotionofapoptosisisheavilydisputed,sincedatasupportingbothviewshavebeenreportedinseveralindependentstudies.Toclarifythisissue,andalsotodeterminewhetherclusterinexpressionitselfmightbeaffectedbyapoptosis,inthepresentstudy,ratthymocytesweretreatedwithdexamethasone,-asyntheticglucocorticoidthatelicitsapoptosisinthymocytes-,andclusterinmRNAexpressionwasanalyzedbysemi-quantitativeRT-PCRbeforeandafterinductionofapoptosis.Interestingly,neitherthetreatmentwithdexamethasoneinvitronortriggeringofapoptosisinvivoup-regulatedclusterinexpression,opposingtheviewthatclusterinisinvolvedinapoptoticprocesses.Ontheotherhand,anewclusterinmRNAisoformwasdetectedandisolated,whoseexpressionwasrestrictedtofreshlyisolatedthymocytes.Thisnovelisoformlacksthepost-translationalproteolyticcleavagesiteandisthereforepredictedtoencodeamonomericprotein.Thebiologicalfunctionundernormalcircumstances,however,willneedfurtherinvestigationsforclarification.Whileapoptosiscouldnotmodulateclusterinexpression,activationofthymocyteswithconcanavalinAandinterleukin-2resultedinup-regulationofclusterinmRNAlevel,indicatingthatclusterinexpressionisratherunderthecontrolofcellactivation-mediatedratherthanapoptosis-inducedsignals.

简介：InordertoanalyzethemechanismofimmunomodulationbyLPSonmurineperitonealsuppressormacrophages,wehave,usingRNaseprotectionassay,checkedthechangesofmRNAexpressionpatternofseveralcytokinegenesduringtheimmuno-modulation.Ithasbeenfoundthat,aftertreatingperitonealsuppressormacrophageswithLPS,mRNAsofIL-12p35,IL-12p40,IL-6andIFN-γarenewlyappeared,whilethoseofIL-1α，IL-1βandIL-1Raareincreasebandthoseofothercytokines,likeTGF-β1andMIFarenotchangedatall.Itseemscertainthatthosecytokines,whoseexpressionisincreasedbyLPSstimulation,mayberesponsibleforthefunctionalchangesofsuppressormacrophagesduringimmuno-modulation.Amongthesechanges,theappearanceofIL-12mRNAmayplayacriticalrole,and,inthisregard,thesynergeticeffectbetewwnIFN-γandLPSontheincreaseofIL-12p35andIL-12p40mRNAexpressionisaninterestingfinding.

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简介：我们以前报导了人的ACAT1基因通过从染色体抄录的二不连续的RNA处理的interchromosomal生产妄想的mRNA1和7。妄想的mRNA把AUG1397-1399和GGC1274-1276用作翻译开始codons分别地，与在人的房间是确实地在场的后者生产正常50-kDaACAT1和新奇酶地活跃的56-kDaisoform，包括人的导出单核白血球的巨噬细胞。在这个工作，我们报导位于GGC1274-1276codon的附近的RNA第二等的结构为56-kDaisoform的生产被要求。三预言的茎环(nt1255-1268，1286-1342和1355-1384)的效果被transfecting表示plasmids个别地测试进包含的房间野类型，删除或变异的茎环序列连接了到一个部分ACAT18月开的读物框架(ORF)或到另外的基因的ORF。表示模式被西方的污点分析监视。我们发现从染色体7的在上游的stem-loop1255-1268和从染色体1的下游的stem-loop1286-1342为56-kDaisoform的生产被需要，而从染色体1的最后stem-loop1355-1384是非必需的。用有一个稳定的发卡的monocistronic和bicistronic向量的实验的结果证明从GGC1274-1276codon的那翻译开始被一个内部核糖体入口地点(怒火)调停。进一步的实验表明从GGC1274-1276codon的那翻译开始要求在上游的组成AU的RNA第二等的结构和下游地GC富有的结构。这个机械学的工作为人的ACAT1抄本的妄想的性质的生物意义提供进一步的支持。