简介:采用荧光光谱法研究了水溶液中四溴荧光素、四氯四溴荧光素、二碘荧光素、乙基罗丹明B、健那绿B等5种荧光染料探针与牛血清白蛋白(BSA)的相互作用.实验表明,这5种染料探针同牛血清白蛋白结合时,疏水作用力起决定性作用,静电力起次要作用,相比之下牛血清白蛋白(BSA)结合阴离子的能力最强,其次为中性分子,最后为阳离子.通过疏水作用力,5种染料均是以非极性苯基进入BSA疏水性腔体中同色氨酸残基发生作用,弱氢键的形成加强了这种作用力,且使得光谱间能量转移效率明显提高.5种染料探针的极性部位由于极性和空间效应的原因难以进入腔体内部,致使反应均按接近1∶1的方式进行.
简介:DrugsSPD-304(6,7-dimethyl-3-{[methyl-(2-{methyl-[1-(3-trifluoromethyl-phenyl)-1H-indol-3-ylmethyl]-amino}-ethyl)-amino]-methyl}-chromen-4-one)andzafirlukastcontainacommonstructuralelementof3-substitutedindolemoietywhichcloselyrelatestoadehydrogenatedreactioncatalyzedbycytochromeP450s(CYPs).ItwasreportedthatthedehydrogenationcanproduceareactiveelectrophilicintermediatewhichcausetoxicitiesandinactivateCYPs.DrugL-745,870(3-{[4-(4-chlorophenyl)piperazin-1-yl]-methyl}-1H-pyrrolo2,3-β-pyridine)mighthavesimilareffectsinceitcontainsthesamestructuralelement.Weusedmoleculardockingapproachcombinedwithmoleculardynamics(MD)simulationtomodelthree-dimensional(3D)complexstructuresofSPD-304,zafirlukastandL-745,870intoCYP3A4,respectively.Theresultsshowthatthesethreedrugscanstablybindintotheactivesiteandthe3-methylenecarbonsofthedrugskeepareasonablereactivedistancefromthehemeiron.ThecomplexstructureofSPD-304-CYP3A4isinagreementwithexperimentaldata.Forzafirlukast,thecalculationresultsindicatethat3-methylenecarbonmightbethedehydrogenationreactionsite.DockingmodelofL-745,870-CYP3A4showsapotentialpossibilityofL-745,870dehydrogenatedbyCYP3A4at3-methylenecarbonwhichisinagreementwithexperimentinvivo.Inaddition,residuesinthephenylalanineclusteraswellasS119andR212playacriticalroleintheligandsbindingbasedonourcalculations.ThedockingmodelscouldprovidesomecluestounderstandthemetabolicmechanismofthedrugsbyCYP3A4.
简介:以化学沉淀法制备单相的铕离子掺杂硼铝酸盐红色荧光粉YAl3(BO3)4:Eu3+,考察了焙烧温度、掺铕量等因素对材料性能的影响,用X射线衍射、扫描电镜、激发光谱和发射光谱对荧光粉的结构、形貌和发光性能进行了表征.以尿素为沉淀剂,900℃焙烧沉淀前驱体可得到单相荧光粉YAl3(BO3)4:Eu3+,反应温度比传统高温固相法降低了300℃;沉淀法制备的荧光粉粒径分布范围小,无团聚现象,粒径约300nm.掺铕量为10%(物质的量比)时发光强度最大.在260nIn的紫外光激发下,Eu3+的5D0→F72的电偶极跃迁最强,发射光为618nm的红光.
简介:Theperovskite-typeAgTaO3crystalswerepreparedbymildhydrothermalmethodanddeterminedbypowderX-raydiffraction.RietveldrefinementindicatesthatAgTaO3crystallizedinanorthorhombicsystemwiththespacegroupPcmn.Thelatticeparametersarea=5.5822(1)nm,b=7.8522(2)nmandc=5.5347(1)nm,withα=β=γ=90.0o.Thecompoundwascharacterizedbyscanningelectronmicroscopy(SEM),X-rayphotoelectronspectroscopy(XPS),highresolutiontransmissionelectronmicroscopy(HR-TEM)andUV-Visdiffusereflectancespectrometry(UV-VisDRS).ThephotocatalyticactivityofAgTaO3powderwasevaluatedbythedegradationofCongoredunderUV-lightirradiation.Theresultshowsthatthetitledcompoundhasahighphotocatalyticactivityatroomtemperatureandpotentialapplicationinphotocatalysis.
简介:运用多种方法、多种基组对PF(X3∑-)的平衡结构进行优化计算.用QCISD/6—311G(df)方法得到的平衡结构为RPF=0.1589nm,与实验值RPF=0.15897nm进行比较,最为接近,得出QCISD/6—311G(d,)基组为最优基组;然后对PF(X3∑-)进行单点能扫描计算,用正规方程组拟合Murrell-Sorbie函数,得到相应电子态的势能函数解析式,由势能函数计算了与PF(X3∑-)态相对应的光谱常数,结果与实验数据较为一致.这些数据为反应动力学提供了理论依据.
简介:Fe2O3/activecarbon(Fe2O3/AC)nanocompositeswerereadilyfabricatedbypyrolyzingFe3+impregnatedactivecarboninanitrogenatmosphere.Theas-preparedcompositeswerestudiedbyX-raypowderdiffraction(XRD),X-rayphotoelectronspectroscopy(XPS)andtransmissionelectronmicroscopy(TEM).Thecapacitivepropertyofthecompositeswasinvestigatedbycyclicvoltammetry(CV)andgalvanostaticcharge-dischargetest.Physicalcharacterizationsshowthattheγ-Fe2O3finegrainsdispersedintheACwell,withameansizeof21.24nm.Electrochemicaltestsin6mol/LKOHsolutionsindicatethattheas-preparednanocompositesexhibitedimprovedcapacitiveproperties.Thespecificcapacitance(SC)ofFe2O3/ACnanocompositeswasupto188.4F/gthatwasderivedfrombothelectrochemicaldouble-layercapacitanceandpseudo-capacitance,whichwas78%largerthanthatofpristineAC.AsymmetriccapacitorwithFe2O3/ACnanocompositesaselectrodeshowedanexcellentcyclingstability.TheSCwasonlyreducedbyafactorof9.2%after2000cyclesatacurrentdensityof1A/g.
简介:在模拟人体生理条件下,采用紫外光谱法、荧光光谱法、DNA热变性及黏度法研究欧前胡素及同分异构体异欧前胡素与DNA的作用机制,并探讨其构效关系.紫外光谱表明,加入DNA后,欧前胡素和异欧前胡素的紫外光谱均呈现减色效应;荧光光谱显示,随着欧前胡素或异欧前胡素浓度的增大,DNA—BR的荧光被猝灭,表明欧前胡素和异欧前胡素对BR与DNA的结合存在竞争性抑制;盐效应、DNA热变性温度、黏度法等实验进一步证明欧前胡素和异欧前胡素与DNA的作用模式均为嵌插与静电混合作用模式.研究表明,欧前胡素和异欧前胡素均与DNA发生作用,且欧前胡素与DNA作用强于异欧前胡素.