简介:AnHPLCmethodforquantitativedeterminationofadrenalinehydrochlorideinjectionandnoradrenalinebitartrateinjectionwasestablishedandvalidatedwithasubstituteforthereferencesubstance.Phenylephrinehydrochloridewasselectedasthesubstituteforthereferencesubstanceofadrenalineandnoradrenalinebitartrate.Thecorrectionfactorofphenylephrinehydrochloridewithrespecttothereferencesubstanceofadrenalineandnoradrenalinebitartratewasdeterminedunderdefinedconditions.Adrenalinehydrochlorideinjectionandnoradrenalinebitartrateinjectionwerequantifiedbyassayingphenylephrinehydrochlorideandacorrectfactor.TheresultsindicatethattheHPLCmethodwiththesubstituteforreferencesubstancewasreliableandfeasibleforquantitativedeterminationofdrugs.