简介：Thepresentstudywasdesignedtoestablishamulti-wavelengthquantitativefingerprintingmethodforSan-HuangTablets(SHT),awidelyusedandcommerciallyavailableherbalpreparation,wherehighperformanceliquidchromatography(HPLC)withadiodearraydetector(DAD)wasemployedtoobtainthefingerprintprofiles.Asimplelinearquantitativefingerprintmethod(SLQFM)coupledwithmulti-ingredientsimultaneousdeterminationwasdevelopedtoevaluatethequalityconsistencyofthetestedsamplesqualitativelyandquantitatively.Additionally,thecomponent–activityrelationshipbetweenchromatographicfingerprintsandtotalradical-scavengingcapacityinvitro(asassessedusingthe1,1-diphenyl-2-picrylhydrazyl(DPPH)assay)wasinvestigatedbypartialleastsquaresregression(PLSR)analysistopredicttheantioxidantcapacityofnewsamplesfromthechromatographicfingerprintsandidentifythemainactiveconstituentsthatcanbeusedasthetargetmarkersforthequalitycontrolofSHT.Inconclusion,thestrategydevelopedinthepresentstudywaseffectiveandreliable,whichcanbeemployedforholisticevaluationandaccuratediscriminationforthequalityconsistencyofSHTpreparationsandothertraditionalChinesemedicine(TCM)andherbalpreparationsaswell.