简介：Inthepresentstudy,theeffectsofPleurotusnebrodensispolysaccharide(PN-S)ontheimmunefunctionsofimmunosuppressedmiceweredetermined.Theimmunosuppressedmousemodelwasestablishedbytreatingthemicewithcyclophosphamide(40mg/kg/2d,CY)throughintraperitonealinjection.TheresultsshowedthatPN-SadministrationsignificantlyreversedtheCY-inducedweightloss,increasedthethymicandsplenicindices,andpromotedproliferationofTlymphocyte,Blymphocyte,andmacrophages.PN-SalsoenhancedtheactivityofnaturalkillercellsandincreasedtheimmunoglobulinM(IgM)andimmunoglobulinG(IgG)levelsintheserum.Inaddition,PN-Streatmentsignificantlyincreasedthephagocyticactivityofmouseperitonealmacrophages.PN-Salsoincreasedthelevelsofinterleukin-6(IL-6),tumornecrosisfactor-α(TNF-α),interferon-γ(INF-γ),andnitricoxide(NOS)insplenocytes.qRT-PCRresultsalsoindicatedthatPN-SincreasedthemRNAexpressionofIL-6,TNF-α,INF-γ,andnitricoxidesynthase(iNOS)inthesplenocytes.TheseresultssuggestthatPN-Streatmentenhancestheimmunefunctionofimmunosuppressedmice.Thisstudymayprovideabasisfortheapplicationofthisfungusinadjacentimmunopotentiatingtherapyagainstcancerandinthetreatmentofchemotherapy-inducedimmunosuppression.

简介：AnovelPleurotusnebrodensispolysaccharide(PN-S)waspurifiedandcharacterized,anditsimmune-stimulatingactivitywasevaluatedinRAW264.7macrophages.PN-SinducedtheproliferationofRAW264.7cellsinadose-dependentmanner,asdeterminedbytheMTTassay.AfterexposuretoPN-S,thephagocytosisofthemacrophageswassignificantlyimproved,withremarkablechangesinmorphologybeingobserved.FlowcytometricanalysisdemonstratedthatPN-SpromotedRAW264.7cellstoprogressthroughSandG2/Mphases.PN-Streatmentenhancedtheproductionsofinterleukin-6(IL-6),nitricoxide(NO),interferongamma(INF-γ),andtumornecrosisfactor-α(TNF-α)inthemacrophages,withup-regulationofmRNAexpressionsofinterleukin-6(IL-6),induciblenitricoxidesynthase(iNOS),interferongamma(INF-γ)andtumornecrosisfactor-α(TNF-α)beingobservedinadose-dependentmanner,asmeasuredbyqRT-PCR.Inconclusion,theseresultssuggestthatthepurifiedPN-Scanimproveimmunitybyactivatingmacrophages.

简介：目的：探讨陕重楼甾体皂苷类单体A（BM-26）豆甾醇-3-O-β-D-吡哺葡萄糖苷对人肝癌SMMC-7721细胞增殖的影响。方法：将不同浓度单体A（BM-26）与人肝癌SMMC-7721细胞分别在24、48、72h处理后，采用MTT法，测定吸光度A值，并计算细胞增殖抑制率，检测陕重楼甾体皂苷类单体A（BM-26）对肿瘤细胞增殖的影响。结果：不同质量浓度陕重楼甾体皂苷类单体A（BM-26）对人肝癌SMMC-7721细胞均有抑制作用：24h处理时，质量浓度为8、40、200μg·ml^-1，1、5、25mg·mL^-1组与对照绀相比较，吸光度A值有显著性降低（P〈0．01或P〈0．05）；48h处理时，质量浓度为0．3、1．6、8、40、200μg·mL^-1，1mg·mL^-1组与对照组相比较，吸光度A值有显著性降低（P〈0．01或P〈0．05）；72h处理时，质量浓度为8、40性g·mL^-1组与对照组相比较，吸光度A值有显著性降低（P〈0．01或P〈0．05）。其中质量浓度为8μg·ml^-1时，在24、48、72h抑制率分别为45％、41％、36％。结论：陕重楼单体A（BM-26）对人肝癌SMMC-7721细胞增殖具有一定的抑制作用，甘‘最蛆抑制质量浓度为8μg·mL^-1，有效抑制质量浓度范围是8～40μg·mL^-1。