简介：ThepresentstudywasdesignedtoisolateandcharacterizeapurifiedextractfromFusariumsolaniFG319,termedMFS(MetaboliteofFusariumsolaniFG319)thatshowedanti-atherosclerosisactivitybyinhibiting3-hydroxy-3-methylglutarylcoenzymeA(HMG-CoA)reductase.Responsesurfacemethodology(RSM)wasemployedtoachieveanimprovedyieldfromthefermentationmedium.Theinhibitingeffectoftheisolate,MFS,onHMG-CoAreductasewasgreaterthanthatofthepositivecontrol,lovastatin.TheaveragerecoveryofMFSandtherelativestandarddeviation(RSD)rangedbetween99.75%to101.18%,and0.31%to0.74%,respectively.TheRSDsintra-andinter-assayofthethreesamplesrangedfrom0.288%to2.438%,andfrom0.934%to2.383%,respectively.FromtheRSM,theconcentrationofinducer,cultivationtime,andculturetemperatureshadsignificanteffectsontheMFSproduction,withtheeffectofinducerconcentrationbeingmorepronouncedthatotherfactors.Inconclusion,theoptimalconditionsfortheMFSproductionwereachievedusingRSMandthatMFScouldbeexploredasananti-atherosclerosisagentbasedonitsabilitytoinhibitHMG-CoAreductase.