简介:PolysaccharidesfromnumeroustraditionalChinesemedicineshavebeenprovenasthebioactiveingredientsandarehenceusedasthequalitycontrolmarkers.However,theassessmentcriteriaalwaysshowapoorspecificity,duetothelackofmaticcomparisonamongtheanalogousherbs.Inthepresentstudy,twosimilarmaterials,namelysea-tangleandsargassum,wereselectedasthemodelherbstodevelopmorespecificmethodsforqualitycontrol.Twowell-establishedmethods,determinationofthetotalpolysaccharidescontentandmonosaccharidescompositionanalysis,werebothemployed.Baseduponthequantitativeresults,theevaluationcriteriaofthepolysaccharidescontentsofnotlessthan2.0%and1.7%wereproposedforsea-tangleandsargassum,tively.NineidenticalmonosaccharidederivativesappearedontheHPLCchromatogramsofthehydrolysisandderivatizedsolutionsofthetwodrugs.Principalcomponentanalysisandorthogonalpartialleastsquaresdiscriminantanalysisusingthepeakareasofmonosaccharidesderivativesasthevariableswereperformed,andtheresultsindicatedthatmannuronicacidandxylosewiththeoppositeconcentrationsinthetwodrugswerethedifferentialcomponents.Adiscriminativecriterionusingthepeakarearatioofthesetwomonosaccharidesderivativeswasproposedforthequalitativeidentification.Inconclusion,amorespecificandquantitativequalitycontrolmethodwasdevelopedforsea-tangleandsargassum.
简介:NocathiacinI,aglycosylatedthiopeptideantibiotic,displaysexcellentantibacterialactivitiesagainstmultidrugresistantbacterialpathogens.Previously,anovelnocathiacinIformulationforintravenousadministrationhasbeensuccessfullydevelopedanditsaqueoussolubilityisgreatlyenhancedforclinicalapplication.ThepurposeofthepresentstudywastoincreasethefermentationtiterofnocathiacinIandreduceoreliminateanalogousimpuritiesbyscreeningthemediumingredientsusingresponsesurfacemethodology.Afterasysmaticoptimization,awater-solublemediumcontainingquality-controllablecomponentswasdevelopedandvalidated,resultinginanincreaseintheproductionofnocathiacinIfrom150to405.8mg·L-1at150-Lscale.Meanwhile,theanalogousimpuritiesexistedinreportedprocessesweregreatlyreducedoreliminated.Usingoptimizedmediumforfermentation,nocathiacinIwithpharmaceuticallyacceptablequalitywaseasilyobtainedwitharecoveryof67%.Inconclusion,theresultsfromthepresentstudyofferapracticalandefficientfermentationprocessfortheproductionofnocathiacinIasatherapeuticagent.
简介:试图在CalophylluminophyllumL为calophyllolide的决心建立一个GCMS方法。并且它在自然的药的质量控制的申请。从CalophylluminophyllumL的方法Methanolic摘录。被准备。Calophyllolide被孤立,净化然后识别了作为为开发和确认的一个化学标记服务根据ICH指南和USP的一个GCMS方法。确定的方法被证明在原则部件分析(PCA)的帮助下在质量控制有用,一种multivariate数据分析技术。结果建议方法靠着质量是选择的光谱deconvoluting软件AMDIS32。线性范围从3.12550g吗??