简介：Toinvestigatetheexpressionofapoptosis-relatedprotein(Fas,FasL,andBcl-2)inthepathogenesisofautoimmunethyroiddisorders(ATDs),immunohistochemicalstainingwasperformedon20Hashimoto'sthyroiditis(HT),20Graves'disease(GD),and20thyroidfollicularadenoma(TFA,ascontrol).AllthecasesexpressedFas,mainlyonthecellsurfaceandcytoplasm.FasLwasfoundin17casesoftheTFA.Bcl-2wasdetectedin15casesofHT,19ofGDand17ofTFA.InTFA,amoderateFasexpressionandaminimalornoFasLexpressionwasdetectedonfollicularcells.InHT,thefolliclesadjacenttoinfiltratinglymphocytesshowedincreasedlevelsofFasandFasLexpression.AweakerstainingofFasandFasLwasexhibitedoninfiltratinglymphocytesthanonthyrocytes.InacomparisonofGDwithHT,thyrocytesandlymphocytesshowedsimilarFasstaining,butforFasLthestainingwasratherweakerinHT.TheexpressionofBcl-2wasnearlyidenticalinGDandTFA,butmuchweakeronthefollicularcellsinvicinityoflymphocytesandonthelymphocyteslocatedingerminalcentersofHTtissues.TheexpressionofFas,FasL,Bcl-2inHashimoto'sthyroiditisandGraves'diseasewerealmostsame.FasLstrongexpressionandBcl-2weakexpressiononthefolliclesinHTmayinduceapoptosis.TheseresultsprovidedevidenceforexpressionofFas,FasLandBcl-2inthepathogenesisofautoimmunethyroiddisease.ThelymphocytesseemnottobedirectlyengagedintheprocessviatheirownFasL,buttheymayprovidesomecytokinesthat,inturn,upregulateFasand/orFasLexpressiontoinduceapoptosis.

简介：IthasbeenshownthatIL-2andIL-15canhaveopposingeffectsonlifeanddeathofTcells.However,theroleofIL-2andIL-15inregulatingthefateofothercelltypesislessclear.Inthepresentstudy,weexaminedtheimpactofIL-2andIL-15onlifeanddeathofpre-BcellsusingtheBAF-B03line.WeshowedthatBAF-B03cellsconstitutivelyexpressedtheprivateIL-2RαchainandIL-15Rαchain,andthesharedIL-2Rβchainandγcchain.StimulationofBAF-B03cellsinvitrowithIL-2andIL-15inducedvigorouscellproliferationinadose-dependentfashion.TitrationofIL-2andIL-15intheassayshowedthatthemitoticeffectsofIL-2andIL-15wereremarkablysimilar,Howerer,thesensitivitiesofBAF-B03cellstoFasmediatedapoptosisafterIL-2andIL-15stimulationwerestrikinglydifferent.CellsculturedinIL-2readilyunderwentapoptoticcelldeathuponcross-linkingoftheFasreceptorwhereascellsculturedinIL-15wereextremelyresistanttoFastriggeredcelldeath.Theanti-apoptoticeffectofIL-15inthismodelwasassociatedwithincreasedexpressionofBcl-xL.FLIPexpression,however,wascomparablebetweenIL-2andIL-15stimulatedcells.WeconcludethatIL-2andIL-15havediametricallyoppositeeffectonthefateofBAF-B03cells,althoughbothcytokinessharesimilarreceptorstructureandexhibitsimilarmitoticactivities.

简介：TodeterminetheregulatoryeffectsofestrogenandcytokineIL-6andIL-8onthegrowthofepithelialovariancancer(OVCA),wefirstexaminedthestatusofestrogenreceptors(ERαandERβ),IL-6receptor(IL-6Rαandgp130),andIL-8receptor(IL-8RAandIL-8RB)onfiveepithelialOVCAcelllinesbysemiquantitativeRT-PCRandWesternblotanalysis.Resultsshowedthattheexpressionsofthesereceptorswerevariableonthefivecells.ThoseOVCAcellsexpressingthereceptorswereselectedtostudyrelatedmolecularmechanism.MTTassaywasperformedtoobservetheeffectsof17β-estradiol(E2),IL-6andIL-8oncellproliferation.WediscoveredthatE2markedlypromotedtheproliferationofCAOV-3andOVCAR-3cellinatime-anddose-dependentmanner.Tamoxifen(Txf),anERinhibitor,completelyblockedtheproliferationoftheE2-inducedcells,andIL-6-or/andIL-8-neutralizingantibodyonlyshowedpartiallyblockingactivity.IL-6andIL-8wereabletosignificantlystimulateCAOV-3andOVCAR-3cellproliferationinatime-anddose-dependentmanner,whichhadapotentialsynergisticeffectonCAOV-3cellsbutnotonOVCAR-3cells.Thecellproliferationinducedbythesetwocytokineswasabolishedcompletelybytheirspecificneutralizingantibodies,partiallybyTxf,butnotbyunrelatedgoatIgG.Takentogether,ourresultssuggestedthatestrogen,IL-6andIL-8couldmodulateOVCAgrowthbyformingareciprocalcascadewithamplifyingeffect.Cellular&MolecularImmunology.

简介：到病原体的暴露导致象巨噬细胞那样的介绍抗原的房间(APC)和树枝状的房间(DC)生产各种各样的内长的调停人，包括导出的arachidonic酸(AA)eicosanoids，cytokines，和氮的氧化物(没有)。激活的APC的许多分泌产品能以一种autocrine方式自己行动并且调制他们的功能。而且，在内长的bioactive分子之间跨相互作用为他们激活并且支撑有免疫力、煽动性的回答，并且调整有免疫力的动态平衡的能力与重要后果调整职业APC的函数。尽管为许多忽视了什么时候的年与他们在心血管的动态平衡，癌症和发炎的角色相比，在免疫学的eicosanoids的重要性正在变得更多定义。前列腺素(PG)的角色E2(PGE2)，知道的最好和大多数之一很好学习了eicosanoids，具有特别兴趣。它由对他们分泌cytokines的区别，成熟和他们的能力起作用调制职业DC的活动。特别地在造血的cytokines之中，interleukin-10(IL-10)是显示immunostimulatory和immunoregulatory活动的一个多种的分子。因为它的反煽动性的性质，IL-10依附许多注意。它由myeloid起源的房间调制cytokines，可溶的调停人和房间表面分子的表示，特别地巨噬细胞和DC。我们以前报导PGE2是在骨头的IL-10的有势力inducer导出髓的DC(BM-DC)，和导致PGE2的IL-10是BM-DC支持inflammatory显型的一个关键管理者。BM-DC可以被看作一个重要模型学习在内长的调停人之间的复杂相互作用，并且autocrineIL-10在导出AA的类脂化合物调停人，的crossregulation起一个枢轴的作用cytokines，并且没有，与有免疫力、煽动性的回答上的批评效果。细胞与分子的免疫学。2006；3(4):271-277。

简介：ToaugmentspecificcytotoxicTlymphocyte(CTL)lysisisapromisingstrategyforcancertherapy.Inthisstudy,weexaminedtheboostingeffectofCTLsuponautologouslymphoblastoidBcelllines(LCLs)transfectedwithdiverseplasmids,toexplorethepossibleCTL-basedimmunotherapyofnasopharyngealcarcinoma(NPC).FCManalysisdisplayedratherhighratio(＞30%)ofsuccessfullytransfectedLCLsbyutilizingtheDMRIE-Ckit.CTLassaysdemonstratedthatsubstantiallyhigherratioofCTLspecificlysiswasobservedupontheLCLstransfectedwithbothexpressionvectorsencodingEBV-specificepitopesandtheirpresentationmoleculeHLA-A2,incontrastwiththosetransfectedseparately.BytransfectingthevectorencodingHLA-A2alone,onlytheLCLsofHLA-A2+donorselicitedmarkedlyhigherCTLlysis.CTLassaysalsoshowedthatthereexistednomarkeddifferencesupontransfectionbyeitherdifferentvectors(pcDNA3,pNGVL3orpNGVL3-hFlex),ordifferentEBV-derivedpeptides(LMP2Pep1orLMP2Pep2),orwithorwithoutthedoubledDNAsequenceencodingpeptides.ThisstudyindicatedapromisingimmunotherapystrategyonNPCthroughboostingandelicitingtheEBV-specificCTLactivationbytransferringvectorsencodingbothEBV-specificepitopesandtheirpresentationmoleculeHLA-A2intoautologousLCL,thepresentationcellsofMHC/peptidetetramericcomplex.