简介：Objective:MicroRNA-21(miR-21)hasbeenshowntobeakeyregulatorofcarcinogenesis.TherewerefewreportsaboutthecomparisonofserummiR-21withconventionaltumormarkers.ThisstudyaimedtoexplorethediagnosticvalueofcirculatingmiR-21asatumormarkerinbreastcancer(BC)andcompareitwithCA153andcarcinoembryonicantigen(CEA).Methods:CirculatingmiR-16andmiR-21wereamplifiedandquantitativelydetectedbyreal-timePCRin89BCpatientsand55healthycontrols.ThelevelsofCA153andCEAweremeasuredthroughelectrochemiluminescenceassays.ThenthesensitivityindiagnosisofBCwascomparedamongmiR-21,CA153andCEA.Results:ThelevelofserummiR-21wassignificantlyhigherinBCpatientsthancontrols(P<0.001).ThesensitivityandspecificityofmiR-21were87.6%and87.3%,respectively,whereasthesensitivitiesofCEAandCA153wereonly22.47%and15.73%.Conclusions:ComparedwithCEAandCA153,serummiR-21hasahighersensitivityindiagnosisofBC.AlthoughnotcorrelatedwiththestatusofER,PRandclinicalstages,serummiR-21maybeapotentialdiagnosticindicatorforBC,especiallyfortheearlystage.

简介：Objective:Thisworkaimedtostudythesafetyandefficacyofpreoperativeintestinalstentdecompressioncombinedwithlaparoscopicsurgerytotreatleft-sidedcolorectalcancerwithobstruction(LCCO).Methods:Retrospectiveanalysiswasconductedondataobtainedfrom21LCCOpatientsadmittedtoTheFirstAffiliatedHospitalofZhejiangChineseMedicineUniversityduringMarch2008andDecember2011.Toremovetheintestinalobstruction,preoperativeintestinalstentplacementundercolonoscopicguidancewasperformed.Approximately7to10daysaftertheoperation,laparoscopicradicalsurgeryofcolorectalcancerwasconducted.Results:Amongthe21casesstudied,laparoscopicsurgerywassuccessfulin20patients.Emergentlaparotomywasconductedinonepatientbecauseoftumorinvasionintheureter.Thedurationoftheoperationrangedfrom180to320min,andtheaveragetimewas220min.Therecoverytimeforbowelfunctionrangedfrom2to5dayswithanaveragetimeof3days.Postoperativeinfectionoftheincisionoccurredinonecase.Noanastomoticleakagewasobservedinanyofthecases.Conclusion:Preoperativeintestinalstentdecompression,combinedwithprimarystagelaparoscopicsurgery,isasafeandeffectivemethodforthetreatmentofLCCO.

简介：DBC1是近年来新发现的一种蛋白,属于小GTP酶类Rho家族的非典型成员。最初因为在乳腺癌组织中杂合性缺失被鉴定出来的。目前关于DBC1在乳腺癌上的研究报道尚少。本文对国内外关于该基因的有关研究进行综述,了解其分子机制,基因表达情况。

简介：Theacquisitionofsecondarychromosomalaberrationsinchronicmyeloidleukemia(CML)patientswithPhiladelphiachromosome-positive(Ph+)karyotypesignifiesclonalevolutionassociatedwiththeprogressionofthediseasetoitsacceleratedorblasticphase.Therefore,theseaberrationshaveclinicalandbiologicalsignificance.T(3;12)(q26;p13),whichisarecurrentchromosomalaberrationobservedinmyeloidmalignancies,istypicallyassociatedwithdysplasiaofmegakaryocytes,multilineageinvolvement,shortdurationofanyblasticphase,andextremelypoorprognosis.Wehaveidentifiedarecurrentreciprocaltranslocationbetweenchromosomes3and12withdifferentbreakpointatbands3q21inthemalignantcellsfroma28-year-oldman.ThepatientwasinitiallydiagnosedashavingPh+CMLinthechronicphase.Thet(3;12)(q21;p13)translocationoccurred4yearsafterthepatientwasfirstdiagnosedwithCMLwhileundergoingtyrosinekinaseinhibitortherapy.Weconfirmedthet(3;12)(q21;p13)translocationviafluorescenceinsituhybridizationassaybyusingwhole-chromosomepaintprobesforchromosomes3and12.Ourfindingsdemonstratethat,similartootherrecurrenttranslocationsinvolving3q26suchast(3;3)andt(3;21),thet(3;12)(q21;p13)translocationisimplicatednotonlyinmyelodysplasticsyndromeandacutemyeloidleukemiabutalsointheprogressionofCML.Thesefindingsextendthediseasespectrumofthiscytogeneticaberration.

简介：脊髓脓肿是少见的腔隙性、化脓性中枢神经系统感染。其成因与脑脓肿相似，感染性渗出诱发以产酶的多核细胞为主的炎症，引起液化性坏死，坏死组织继而被纤维母细胞产生的包囊所围绕。

简介：结肠癌转移相关基因1（metastasis．associatedincoloncancer-1，MACC1）是通过对结肠癌的研究新发现并证实的基因，近年来对MACC1与多种恶性肿瘤的相关研究逐渐受到关注，并取得一定成果，本文就此进行综述。

简介：Objectiveandbackground:Althoughp21rashasbeenreportedtobeupregulatedinhepatocellularcarcinomacomplicatingchronichepatitisCtypeI,p21rashasadifferentroleinadvancedstages,asithasbeenfoundtobedownregulated.Thegoalofthisstudywastoinvestigatethestatusofp21rasinearly-stage/low-gradeandlate-stage/high-gradehepatocellularcarcinomaanditspossiblelinktoapoptosis.Materialandmethods:Thirty-fivecaseseachofchronicHCVhepatitistype4(groupI)andcirrhosiswithhepatocellularcarcinoma(HCC)complicatingchronicHCVhepatitis(groupsIIandIII)wereimmunohistochemicallyevaluatedusingap21raspolyclonalantibody.Theapoptoticindexwasdeterminedinhistologicsectionsusingtheterminaldeoxynucleotidyltransferase-mediatedd-UTPbiotinnickendlabeling(TUNEL)assay.Results:Significantdifferences(P=0.001)weredetectedinp21rasproteinexpressionbetweenthethreegroups.Anear2-foldincreaseinp21rasstainingwasobservedinthecirrhoticcasescomparedtothehepatitiscases,andp21rasexpressionwasdecreasedintheHCCgroup.p21rasexpressioncorrelatedwithstage(r=0.64,P=0.001)andgrade(r=-0.65,P=0.001)intheHCCgroupandgradeintheHCVgroup(r=0.44,P=0.008).Bothp21rasexpressionandTUNEL-LIweresignificantlylowerinlargeHCCscomparedtosmallHCCs(P=0.01each).TheTUNELvalueswerenegativelycorrelatedwithstageintheHCCgroup(r=-0.85,P=0.001).TheTUNELvalueswerealsonegativelycorrelatedwithgradeinboththeHCVandHCCgroups(r=0.89,P=0.001andr=-0.53,P=0.001,respectively).Thep21rasscoresweresignificantlycorrelatedwiththeTUNEL-LIvaluesintheHCCgroup(r=0.63,P=0.001)andHCVgroup(r=0.88,P=0.001).Conclusions:p21rasactsasaninitiatorinHCCcomplicatingtype4chronicHCVandisdownregulatedwithHCCprogression,whichmostlikelypromotestumorcellsurvivalbecauseitfacilitatesthedownregulationofapoptosiswithtumorprogression.

简介：一、临床资料患者女,65岁,因'呕血4小时'于2012年11月22日入院。患者于入院前4小时无明显诱因出现呕血,约6次,每次量约100ml,色暗红,发病前患者自觉无明显不适,大便未见异常。该患者于1年前因胃溃疡行全胃切除术,20年前因阑尾炎行阑尾切除术。查体:T38.3℃,P93次/分,R20次/分,BP130/88mmHg,痛苦病容,推入病房,腹部隆

简介：乳腺癌是女性最常见的恶性肿瘤之一，而男性乳腺癌少见，男性乳腺囊内乳头状癌更为罕见。现报道1例男性乳腺囊内乳头状癌。

简介：目的总结探讨腹膜假性黏液瘤的临床特征和诊疗方法.方法报道1例腹膜假性黏液瘤的临床资料,并复习相关文献,总结腹膜假性黏液瘤的临床特征和诊疗方法.结果患者主诉进行性腹胀,腹部CT示腹腔内大量低密度液体,腹部脏器受压体积变小,边缘模糊.患者行剖腹探查肿瘤细胞减灭术及腹腔热灌注化疗,术后病理证实为低级别腹膜假性黏液瘤,随访15个月,患者生活质量良好.结论腹膜假性黏液瘤临床少见,以大量黏液性腹水为特点,具有特征性的影像学表现,治疗方法以反复的肿瘤细胞减灭术为主,辅以腹腔热灌注化疗.

简介：脑胶质瘤为颅内原发的恶性肿瘤，手术切除是主要的治疗方法，术后可出现颅内局部复发或颅内转移，发生颅外转移极为罕见。我院收治1例脑胶质瘤患者，手术后发生脊柱转移，现报告如下。

简介：大脑胶质瘤病（gliomatosiscerebri，GC）是一种罕见的以神经胶质细胞弥漫侵袭性生长为特点中枢神经系统原发性肿瘤．其预后较差．平均中位生存时间为14．5个月。目前大脑胶质瘤暂无标准治疗方案．放化疗联合靶向治疗是治疗该病的有效方法。我科近期收治了一例大脑胶质瘤患者．经联合治疗后效果较好，现报道如下：1病例资料

简介：神经纤维瘤病（neurofibromatosis,NF）是由于基因缺陷导致神经嵴细胞发育异常而引起多系统损害的常染色体显性遗传病。NF虽为良性肿瘤,但可发生恶变和累及其他系统,临床较为少见。福建医科大学附属宁德市医院2012年12月21日收治NFⅠ型伴恶变患者1例。现结合文献复习,报道如下。

简介：骨母细胞瘤是一种少见的成骨性肿瘤，是一种趋向于分化为成骨细胞的良性肿瘤，约占原发性骨肿瘤的1%。骨母细胞瘤好发部位为脊柱和长骨，脊柱骨母细胞瘤一般常见于椎弓根和椎板，可蔓延到椎体，原发于椎体者非常少见。

简介：2012年我科收治了一例肺癌脑转移患者．经综合治疗后目前患者一般情况良好．从确诊脑转移至今已生存20个月。目前脑转移控制问题比较棘手，该病例值得进一步分析探讨。

简介：神经纤维瘤病（neurofibromatosis，NF）义称多发性神经纤维瘤，是源于神经嵴细胞分化异常而导致的多系统损害的常染色体皿性遗传病，亦是常常累及神经、肌肉、骨骼、内脏和皮肤的一种先天性发育不良的疾病。

简介：1临床资料患者,男,71岁,因＂腹泻伴高热,尿中混有粪渣样物半月余＂来我院住院治疗。B超检查示：肝胆脾胰腺及双肾未见占位性病变,右下腹部至膀胱底部可见大小为6.3cm×5.7cm的中等强度光团回声,内可见片状低回声（图1）。

简介：Chronicalcoholconsumptionisamajorriskfactorworldwideaffectingsignificantlybothmortalityandyearsoflifelost(YLL)(1).Ca.5%ofthewesternworldshowriskyalcoholconsumptionandinsomecountriessuchasChinaaregionalyearlyincreaseofalcoholconsumptionofover400%hasbeenobservedrecently(2,3).Theliveris

简介：目的：构建并制备能够有效表达EB病毒BHRF1基因的重组慢病毒载体,观察BHRF1表达对人胚肺成纤维细胞（KMB17）凋亡的影响。方法：采用RT-PCR法,从B95-8细胞扩增EBVBHRF1基因,克隆至pWPIGW慢病毒载体上,与pVSVG及pSPAX质粒共转染人胚肾293T细胞,包装出重组慢病毒。将纯化后的重组慢病毒直接感染293T和KMB17细胞,荧光显微镜、实时定量RT-PCR、免疫印迹等方法检测BHRF1在细胞中的表达水平。流式细胞仪检测BHRF1表达对凋亡诱导剂或无血清培养诱发KMB17细胞凋亡的影响。结果：重组慢病毒介导BHRF1在293T和KMB17细胞内获得表达,能有效地抑制KMB17细胞的凋亡。结论：成功构建了表达BHRF1基因的重组慢病毒载体。

简介：目的探讨PHLPP1蛋白在癌旁正常胃黏膜、胃癌原发灶和淋巴结转移灶中的表达差异,分析PHLPP1蛋白表达与患者临床病理特征及预后的关系。方法应用免疫组化方法检测30例癌旁正常胃黏膜、157例胃癌原发灶和96例淋巴结转移灶中PHLPP1的蛋白表达情况,分析PHLPP1蛋白表达与胃癌临床病理特征的关系及其对患者生存预后的影响。结果患者癌旁正常胃黏膜、胃癌原发灶和淋巴结转移灶中的PHLPP1蛋白阳性表达率分别为:96.7%、61.8%和36.5%,组间差异有统计学意义(P<0.05)。PHLPP1蛋白表达与肿瘤分化程度、T分期、N分期及TNM分期显著相关,与患者年龄、性别、肿瘤大小、术前CEA水平无关。PHLPP1蛋白阳性表达患者总体5年总体生存率为68%,显著高于阴性表达患者34%(P=0.001)。Cox多因素回归分析结果表明T分期、N分期、PHLPP1蛋白低表达是胃癌的独立预后因素(P=0.027)。结论胃癌组织中PHLPP1蛋白缺失表达可能对胃癌发生、发展起促进作用。PHLPP1蛋白阴性表达可作为胃癌患者预后不佳的指标。