简介：Objective:TodetectdifferentialproteinexpressioninmalignantandnormallivercelllinesinvitrousingtheSELDIProteinChipplatform,forinvestigatingthepathogenesisoflivercancer.Methods:Twocelllines,humannormallivercelllineL02andhepatomacelllineSMMC-7721wereculturedroutinely,harvestedingoodconditionandlysed.Afterquantification,thesupernatantofthelysatewastestedbyIMAC3(ImmobilizedMentalAffinityCapture)andWCX2(WeakCationExchange)chipsontheSELDI-TOF-MSProteinChipreader.Results:Proteinexpressiondifferedbetweenthemalignantandnormallivercelllines.Atotalof20differentiallyexpressedproteinswerefound,amongwhich,7werecapturedbytheIMAC3chipand14bytheWCX2chip.Peaksat5,419,7,979and11,265Dawerehigherandat8,103,8,492,10,160and11,304DalowerinSMMC-7721cellsbytheIMAC3chip;peaksat7,517,7,945and7,979Dawerehigherandat5,061,5,551,5,818,7,439,9,401,10,100,10,312,11,621,11,662,11,830and12,772DalowerinSMMC-7721cellsbytheWCX2chip.Interestingly,bothchipscapturedthe7,979Dapeak.Inaddition,the11,081DapeakcorrespondedpreciselywiththemolecularmassofthecalciumbindingproteinS100A10,whichmayparticipateintheformationoflivercancerinassociationwithp36.Conclusion:DetectingdifferentialproteinexpressioninmalignantandnormallivercelllinesusingtheSELDIProteinChipplatformwassimple,sensitiveandrepeatable.Theresultsweobtainedcanserveasabasisforinvestigatingthepathogenesisoflivercancerandaidthediscoveryofnewtherapeutictargets.