简介：Objective:ToestablisharapidandsimpleassayforthediagnosisofChlamydiatrachomatis(CT)infection.MethodsBALB/cmicewereimmunizedwithSDS-PAGEpurifiedmajoroutermembraneprotein(MOMP)fromCTandthemonoclonalantibodieswereobtainedsubsequently.Two-siteELISAwasdevelopedtodetectCTinfection.Results:Theestablishedassaywasabletodetectaslowas1.248ug/mlMOMPwithinterrunandinrunCV6.9%and3.1%respectively.94%(34/36)ofculture-positivesampleswerefoundtobepositiveinthecurrentexamination,indicatingthehighsensitivityofthisassay.Conclusion:TheassayisapplicableforclinicaldiagnosisofCTinfection.
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