简介：Excessivenitrogen(N)fertilizerapplicationtowinterwheatisacommonproblemontheNorthChinaPlain.TodeterminetheoptimumfertilizerNrateforwinterwheatproductionwhileminimizingNlosses,fieldexperimentswereconductedfortwogrowingseasonsateightsites,inHuiminCounty,ShandongProvince,from2001to2003.TheoptimumNrateformaximumgrainyieldwasinverselyrelatedtotheinitialsoilmineralNcontent(Nmin)inthetop90cmofthesoilprofilebeforesowing.TherewasnoyieldresponsetotheappliedNatthethreesiteswithhighinitialsoilmineralNlevels(average212kgNha-1).TheaverageoptimumNratewas96kgNha-1forthefivesiteswithlowinitialsoilNmin(average155kgNha-1)beforesowing.ResidualnitrateNinthetop90cmofthesoilprofileafterharvestincreasedwithincreasingfertilizerNapplicationrate.TheapparentNlossesduringthewheat-growingseasonalsoincreasedwithincreasingNapplicationrate.TheaverageapparentNlosseswiththeoptimumNrateswerelessthan15kgNha-1,whereasthefarmers'conventionalNapplicationrateresultedinlossesofmorethan100kgNha-1.Therefore,optimizingNuseforwinterwheatconsiderablyreducedNlossestotheenvironmentwithoutcompromisingcropyields.

简介：Changesofsoilorganicnitrogenformsandsoilnitrogensupplyundercontinuousricecroppingsystemwereinvestigatedinalong-termfertilizationexperimentinJinhua,ZhejiangProvince,China.ThefertilizertreatmentsincludedcombinationofP-K,N-K,N-P,andN-P-Kaswellasthecontrol.Aftersixyearsofcontinuousdouble-ricecropping,totalsoilNandhydrolysableNcontentsremainedstableinplotswithNtreatments,whilethehydrolysableNcontentsweresubstantiallyreducedinthoseplotswithoutNapplication.Comparedtotheunbalancedfertilizationtreatments,PandKincreasedthepercentageofhydrolysableammoniumNinthetotalsoilNwiththebalancedapplicationofN,andalsomaintainedhigherricegrainyieldsandnitrogenuptake.GrainyieldwaspositivelycorrelatedwithtotalNuptake(r=0.875**),hydrolysableN(r=0.608**),hydrolysableammoniumN(r=0.560**)andthehydrolysableunknownN(r=0.417**).TotalNuptakewaspositivelycorrelatedwithhydrolysableN(r=0.608**),hydrolysableammoniumN(r=0.440**)andhydrolysableunknownN(r=0.431**).Soilnutrientdepletionand/orunbalancedfertilizationtoricecropreducedNcontentinsoilmicrobialbiomass,andthereforeincreasedC/Nratio,suggestinganegativeeffectonthetotalmicrobialbiomassinthesoil.

简介：PotexperimentswerecarriedouttoestimateN2fixationbyvetch,milkvetch,sicklealfalfaandbroadbeaninpurestandusinga^15N-labelledsoil.Winterwheatwasusedasthenon-fixingcontrol.The15N-labelledsoilusedwaspreparedbygrowingcorn-wheat-cornsuccessivelyonanearlyorganic-matter-freeXiashuloesssupplementedwithadequateamountsof(15NH4)2SO4,P,Kandmicronutrients,thenincorporatingthese15N-labelledplantmaterialsintothesoilaftereachhavest,andallowingtheplantmaterialstobedecomposedaerobicallyfor410dafterincorporationoftheplantmaterialofthethirecrop.The15Nenrichmentofwheatplant-Nvariedslightlywithorgans,withamaximumdifferenceof9.8%,Basedon15NenrichmentofsoilNinferredfromthemeanvalueofthe15Nenrichmentindifferentorgansofwheat79%-91%oftotalNinthetopsand67%-74%oftotalNintherootsoflegumesstudiedwerederivedfromatmosphere.EstimatebyisotopedilutionmethodwasingoodagreementwiththatbytheconventionaldifferencemethodprovidedvaluesobtainedbythelatterwerecorrectedforseedN,andalsowiththatfromthemeasurementofNaccumulatedinthetopsofthelegumes.

简介：Fivehumicfractionswereobtainedfromauniformly^15N-labelledsoilbyextractionwith0.1molL^-1Na4P2O7,0.1molL^-1NaOH,andHF/HCl-0.1molL^-1NaOH,consecutively,andanalyzedby^13Cand^15NCPMASNMR(crosspolarizationandmagicanglespinningnuclearmagneticresonace).Comparedwiththoseofnativesoilshumicfractionsstudiedasawholecontainedmorealkyls,methoxylsandO-alkyls,being27%-36%,17%-21%and36%-40%,respectively,butfeweraromaticsandcarboxyls(bein14%-20%and13%-90%,respectively),Amongthosehumicfractions,thehumicacid(HA)andfulvicacid(FA)extractedby0.1molL^-1Na4P2O7containedslightlymorecarboxylsthancorrespondinghumicfractionsextractedby0.1molL^-1NaOH,andtheHAextactedby0.1molL^-1NaOHaftertreatmentwithHF/HClcontainedtheleastaromaticsandcarboxyls.Thedistributionofnitrogenfunctionalgroupsofsoilhumicfractionsstudiedwasquitesimilartoeachotherandalsoquitesimilartothatofhumicfractionfromnativesoils.Morethan75%oftotalNineachfractionwasinamidefrom,with9%-13%presentasaromaticand/oraliphaticaminesandtheremainderasheerocyclicN.

简介：ExperimentswerecarriedoutonavegetablefieldwithPekingcabbage(Brassicapekinensis(Lour.)Rupr.),cabbage(Brassicachinensisvar.OleiferaMakinoandnemoto),greencabbage(BrassicachinensisL.),spinach(SpinaciaoleraceaL.)andrape(BrassicacampestrisL.)tostudytheeffectsofNformsandNratesontheirgrowthandnitrateaccumulation.Theresultsindicatedthatapplicationofammoniumchloride,ammoniumnitrate,sodiumnitrateandureasignificantlyincreasedtheyieldsandnitrateconcentrationsofPekingcabbageandspinach.Althoughnosignificantdifferencewasfoundintheyieldsafterapplicationofthe4Nforms,nitrateNincreasednitrateaccumulationinvegetablesmuchmorethanammoniumN.ThevegetableyieldswerenotincreasedcontinuouslywithNrateincrease,andoversupplyofNreducedtheplantgrowth,leadingtoayielddecline.Thistrendwasalsotruefornitrateconcentrationsinsomevegetablesandatsomesamplingtimes.However,asawhole,thenitrateconcentrationsinvegetableswerepositivelycorrelatedwithNrates.Thus,additionofNfertilizertosoilwasthemajorcauseforincreasesinnitrateconcentrationsinvegetables.Nitrateconcentrationsweremuchhigherinroots,stemsandpetiolesthaninbladesatanyNrate.

简介：Aerobicstaticpilecomposting(mechanicalturningevery3days)ofpigmanurewaspreparedat8m3windrowheaps.Sawdustwasusedasthebulkingagenttoprovideadditionalcarbonandtoincreasetheporosityofthesubstrate.TwotreatmentsatinitialC/Nratiosof30and15,respectively,weredesignedinthestudy.Dissolvedorganiccarbon(DOC),solubleNH4+-N,C/Nratiosinsolidandaqueousphases,E4/E6ratios,andseedgerminationindex(GI)weredeterminedtoevaluatethematurityoftheco-composts.Seedgerminationindex,abiologicalparameter,wassuggestedasoneofthemostreliablematurityindicatorsfororganiccompost.TheresultsshowedthatthetreatmentattheinitialC/Nratioof30reachedmaturityafter49daysofcomposting;however,thetreatmentattheinitialC/Nratioof15shouldrequirecompostingtimeoflongerthan63daystoobtainmaturation.Chemicalmulti-indicatorevaluationwasnecessary,andtheGImeasurementwastherecommendedapproachformaturityevaluationinthestudy.

简介：FieldlysimetermethodwasemployedtoinvestigatethedownwardmovementandleachingofNappliedtosummercorn(ZeamayL.)ondrylandsoilinBeijing,AN-fertilizer(120kgNhm^-2)andacontroltreatmentwerarrangedforthestudy.Soilsolutionwascollectedatdepthsof20,40,60,120and170cm,whileleachtewasollectedatthebottom(220cm)ofthelysimeter,TheresultsshowedthatthedownwardmovementofNO3^-Ninsoilprofilewasgreatlyaffectedbyrainfallpattern.ThepeakofleachedNO3^-Nfrombothtreatmentscoincidedwiththepeakoftherainfall.Inaddition,leachedNO3^-Nfrombothtreatmentsandrainfallweresignificantlycorrelated(P<0.05).TheamountofleachedNO3^-NwasnotgreatintheN-fertilizertreatment,TheresultsalsosuggestedthatNfertilizationcouldcauseNO3^--Ncontaminationofgroundeaterduringtherainyseason.

简介：Nitrogenousfertilisersareunderconsiderationforpromotingthegrowthofnursery-rearedhooppine(AraucariacunninghamiiAitonexA.Cunn)seedlingsintheestablishmentphaseofsecondrotation(2R)plantations.Using15N-labelledfertilisers,weinvestigatedtheeffectofdifferentforms(ammoniumsulphate,ammoniumnitrate,potassiumnitrateandurea)andratesofapplication(0,150and300mgNkg-1driedsoil)offertilisersonthegrowth,15Nrecoveryandcarbonisotopecomposition(δ13C)ofhooppineseedlingsina12-monthglasshousetrialinsoutheastQueensland,Australia.The15N-labelledfertiliserswereappliedtonursery-rearedhooppineseedlings,whichwerethengrowninpots,containingca.1.2kgdriedsoil,underwellwateredconditionsfor12months.Fourseedlingsfromeachtreatmentwereharvestedat4-monthintervals,dividedintoroots,stemandfoliage,withafurthersubdivisionfornewandoldfoliage,andthenanalysedfor15N,totalN,δ13CandtotalC.Therewasnosignificantresponseintheseedlinggrowthtotheformorrateofapplicationofnitrogen(N)fertiliserwithinthe12-monthperiod,indicatingthattheseedlingsdidnotexperienceNdeficiencywhengrownonsecondrotationhooppinesoils.Whilethecombined15Nrecoveryfromsoilandplantremainedataround70%throughouttheexperiment,theproportionof15Nrecoveredfromtheplantsincreasingsteadilyovertime.Nitratecontainingfertilisersat150mgNkg-1soilgraduallyincreasedseedlingfoliageδ13Coverthe12-monthperiod,indicatinganincreaseinseedlingwateruseefficiency.

简介：Natural^15NabundancemethodwasusedinthisstudytoinvestigatetheN2-fiingcapacitiesofseveralherbaceousandshrublegumesbyafieldexperiment,Forherbaceouslegumes,theresultswereinconsistentwiththoseobtainedbyapotexperiment.CrotalariamucronataDesv.hadahigher,N2-fixingcapacitythanVignasinensis(L.)Savi.Forshurublegumes,N2-fixingCapacityunderfieldconditionwasslightlydifferentfromthatinpotexperiment.Theseresultsdemonstratedthatthenatural^15NabundancemethodwasapplicabletoevaluatingN2-fixingcapacitiesofherbaceousandshrublegumes,andthatpotexperimentwassuitableforevaluatingtheN2-fixingcapacitiesofnotonlyherbaceouslegumesbutalsoshrublegumes.LeguminousN2-fixingplantsdifferedinresponsetophosphorusfertilization,Phosphorusfertilizerappicationgreatlyincreasedthepercentageofnitrogenderivedfromair(%Ndfa)andtotalamountofN2fixedbyLespedezformosa(Vog.)Koehne(Jiangxi),PhosphorusfertilizershowednosignifcanteffectontheN2-fixingpercentageofCajanuscajan(L.)Millsp.butincreaseditstotalbiomass,thusincreasingthetotalamountofN2fixed.

简介：AfieldexperimentwasconductedonChinesecabbage(BrassicacampestrisL.ssp.pekinensis(Lour.)Olsson)inaNanjingsuburbin2003.Theexperimentincluded4treatmentsinarandomizedcompleteblockdesignwith3replicates:zerochemicalfertilizerN(CK);ureaatratesof300kgNha-1(U300)and600kgNha-1(U600),bothasbasalandtwotopdressings;andpolymer-coatedureaatarateof180kgNha-1(PCU180)asabasalapplication.Theacetyleneinhibitiontechniquewasusedtomeasuredenitrification(N2+N2O)fromintactsoilcoresandN2Oemissionsintheabsenceofacetylene.ResultsshowedthatcomparedtoCKtotaldenitrificationlossesweresignificantlygreater(P≤0.05)inthePCU180,U300,andU600treatments,whileN2OemissionsintheU300andU600treatmentsweresignificantlyhigher(P≤0.05)thanCK.IntheU300andU600treatmentspeaksofdenitrificationandN2OemissionwereusuallyobservedafterNapplication.Inthepolymer-coatedureatreatment(PCU180)duringtheperiod20to40daysaftertransplanting,higherdenitrificationratesandN2Ofluxesoccurred.Comparedwithurea,polymer-coatedureadidnotshowanyeffectonreducingdenitrificationlossesandN2OemissionsintermsofpercentageofappliedN.Astemperaturegraduallydecreasedfromtransplantingtoharvest,denitrificationratesandN2Oemissionstendedtodecrease.Asignificant(P≤0.01)positivecorrelationoccurredbetweendenitrification(r=0.872)orN2Oemission(r=0.781)fluxdensitiesandsoiltemperatureintheCKtreatmentwithastablenitratecontentduringthewholegrowingseason.

简介：Threegoatswerefedwith^15N-labelledricestrawtostudythecharacteristicsofdigestion,assimilation,transformationandexcretionofCandNcompoundsfromricestraw.Itwasshownthattheamountof^15Ntransformedintothebodiesofthetwoslaughteredgoatsaccountedfor38.5and23.6%ofthetotalamountof^15Ndepositionoftheexperimentaldiettakenbyeachgoat.The^15Nexcretedthroughthefecesandurineforthethreegoatsaccountedfor34.8,33.2and33.9%ofthetotalamountof^15Ndepositioninthefeedofthe3goats.Therecoveryoftotal^15Nforthetwoslaughteredgoatswere73.3and57.5%,withthecorrespondingratesof^15Nloss26.7and42.5%respectively.ThedigestibilitiesoftotalaminoacidsforGoats1and3were68.7and54.0%,andthedigestibiltiesofcarbohydratesforthetwogoatswere74.8and67.7%respectively.

简介：Soilnitrogenpools(NP),denitrification(DN),grossnitrification(GN),N2OandCO2fluxrateswiththeirresponsestotemperatureincreasesweredeterminedunderfivedifferentlandusesandmanagementsinasubalpineforest-grasslandecotoneoftheeasternTibetanPlateau.Landusesconsistedof1)sparsewoodland,2)shrub-land,3)naturalpasture,4)fencedpasture,and5)tilledpasturemimickingagradientdegeneratingecosystemundergrazingimpacts.TheNO3--NcontentwashigherthantheNH4+-Ncontent.Comparingtilledpasturewithfencedpastureshowedthathigherintensivemanagement(tillage)ledtoasignificantdecreaseofsoilorganicmatter(SOM)(P＜0.05)inthesoils,whichwasincontrasttothesignificantincreases(P＜0.05)ofDN,GN,N2OandCO2fluxrates.GN(excludingtilledpasture)andCO2fluxratesincreasedwithatemperaturerise,butDNandN2Ofluxratesnormallyreachedtheirmaximumvaluesat12-14℃withtilledpasture(thehighestmanagementintensity)beingverysensitivetotemperatureincreases.TherewasadifferencebetweennetnitrificationandGN,withGNbeingabetterindicatorofsoilnitrification.

简介：Thechloroformfumigation-incubationmethowasusedtomeasurthesoilmicrobialbiomassC(SMBC)andN(SMBN)in16loessialsoilssampledfromAnsai,YongshouandYanglinginShaanxiProvince.TheSMBContentsinthesoilsrangedfrom75.9to301.0μgCg^-1withanaverageof206.μgCg^-1,accountingfor1.36%-6.24%ofthetotalsoilorganicCwithanaverageof3.07%,andtheSMBNcontentsfrom0.51to68.40μgNg^-1withanaverageof29.4μgNg^-1,accountingfor0.20%-5.65%ofthetotalNinthesoilswithanaverageof3.36%.AcloserelationshipwasfoundbetweenSMBCandSMBN,andtheybothwerepositivelycorrelatedwithtotalorganicC,totalN,NaOHhydrolizableNandmineralizableN.Theseresultsconfirmedthasoilmicrobialbiomasshadacomparativeroleinnutrientcyclesofsoils.

简介：EctomycorrhizalfungusLaccariabicolorS238N,isolatedfromaforestsoilincentralFrancein1990s,hasdemonstratedunequivocallyandabilitytopromotepinegrowth.Inthepresentnurserybedexperiment,theabilityofthisectomycorrhizalfungustoincreasegrowthandPandKuptakeofDouglasFirseedlings(Zone22)wasexamined.Growthofinoculatedseedlingswasovertwice(plantheight)andthreetimes(biomass)thatofnon-inoculatedones.Similarly,boththeconcentrationsandtheamountsofPandKuptakebyseedlingsweresignificantlyincreasedbyfungalinoculation,indicatingtheimprovementofPandKnutritioninmycorrhizalseedlings.Incontrast,Al-Pinthesoilswasdecreasedobviouslybyplants,especiallybymycorrhizas,suggestingutilizationofthissoilPpoolbyplantsandmoreefficientAl-Pmobilizationbymycorrhizasthanbynomycorrhizas.Moreover,Kextractedby1mol/LHClfollowingconsecutiveextractionofH2OandCH3COONH4,whichmaynotbeplantavailable,couldbeutilizedbyfunguscoloniedroots.ThiscouldbeexplainedbythereleaseofprotonsandoxalatebyhypaewhichleadstoreplacementofinterlayerKinnonexpanded2:1claymineralsandbio-weatheringofphyllosilicates.

简介：为探寻温带地区山地植物N素保存策略，找出影响植物N素回收率的环境因子，以便了解植物保持所吸收养分的机制，以小浪底库区山地为例，研究该区域3种生活型21种典型植物成熟绿叶中的N素含量、枯叶中N素含量以及N素回收效率（NRE）。结果表明：植物成熟绿叶中的N素含量和枯叶中N素含量有显著的正相关关系；所测定的21种植物NRE在17，4％～50．1％之间，平均值为28-8％，多数种的回收效率低于40％。测定的21种植物都属于N素回收不完全植物。不同生活型的植物其回收效率有显著的差异，一般表现为草本〉灌木〉乔木。植物叶片N素含量与土壤有机质，上壤N素含量和土壤含水量呈显著正相关；而NRE与土壤有机质、土壤N素含量和土壤含水量呈显著负相关关系。说明在研究区不利的土壤条件，可以导致植物NRE的提高，植物在不利的环境条件下。可以通过提高NRE等途径来应对环境的胁迫。