简介：Bt5198，包含Bt基因的一根新米饭restorer线，与BtMinghui63从精英restorer线Chenghui177的十字和回交被开发，一根转基因的Btrestorer线。生来的线用PCR扩大，测试纸评估，在实验室的昆虫抵抗评估和稻地被评估，大米强风抵抗的托儿所评估和农学的特点的家谱选择。当米饭灰煤杆在实验室与有斑纹的茎borer(SSB)的鸡蛋被接种时，Bt5198和BtMinghui63上的幼虫的死亡是100%。Bt5198在地条件下面对SSB和黄茎borer(YSB)是高度抵抗的。F1混血儿源于Bt5198和四细胞质的男性无菌(厘米)线对SSB和YSB也高度抵抗并且有重要杂种优势。米饭强风抵抗的二年的评估BtMinghui63比那些更好证实到叶的Bt5198的抵抗层次爆炸，颈强风类似于Chenghui177并且显著地的那些。Bt5198的种子萌芽能力和花粉产量与Chenghui177是类似的，建议进新restorer线的Bt基因的介绍没有种子活力或种子生产的收益上的重要效果。识别Bt基因的存在，把测试纸检查与昆虫抵抗的评估相结合是有效的，在实验室并且在地条件下面。

简介：ThetransgenicriceKMD1,expressingasyntheticCry1AbgenefromBacillusthuringiensis,showedeffectiveresistancetoSignificantdeclineswererevealedinfoodconsumptionandgrowthoftheolderRLFnymphsfedonthecut-leavesoftransgenicKMD1plants.TheincreaserateoffoodconsumptionbylarvaefedonKMD1wasdrasticallylowerthanthoseonXiushui11.FoodconsumptionwasvariedwithdifferentinstarswhenthelarvaefedontheBtrice.Thoseoffourth-andfifth-instarlarvaeweredifferentcomparedtothethird-instar,lowerthanthoseonthenon-transgenicricebutstillincreasedalittlewhenthefeedingtimeprolonged.ItisindicatedthatyoungerRLFlarvaearemoresensitivetoBtricethanolderones.Also,about81%,78%and68%ofthethird-,fourth-andfifth-instarRLFlarvaediedwithin72hoursbioassayperiodonKMD1leaves,respectively.TheseresultsdemonstratedthatBt-transgeneinKMD1riceconferssubstantialprotectionagainstinfestationswitholderRLFlarvae.