简介：Molecularmarkershavebeenwidelyusedincropgeneticimprovement,seedtestandgeneticmapping.Ofwhich,sequencecharacterizedamplifiedregion(SCAR)markersareparticularlypopularforitsdiversity,stablereproducibility,andsuitabilityforanalyzinglargenumberofsamples.Inthisstudy,500randomamplifiedpolymorphicDNA(RAPD)primersweretested,andasetofSCARmarkerscomprising37pairsofloci-specificprimersweredevelopedfromtheDNAfragmentsrangingfrom300to1000bpwhichcorrespondtothestable,distinctiveRAPDbandingpatterns.UsingtheseSCARmarkers,59hybridricecombinationswereassessedanddistinguishedinto58subgroupsatthesimilaritycoefficientof0.97inageneticclusteringtreebasedontheallelediversitiesoftheSCARmarkers.Furthermore,13hybridricecombinationswerereassayedwith40randomlyselectedsimplesequencerepeat(SSR)markerstoevaluatetheeffectivenessoftheseSCARmarkers.SSRmarkersproducedsimilarresultstoSCARmarkersasthe13hybridricecombinationswerecompletelyseparatedatthesimilaritycoefficientof0.91intheclusteringtreeestablishedfromSSRpatterns.Takentogether,SCARmarkersprovetobeeffectivetoolsforidentifyinganddifferentiatinghybridricecombinations.