简介:Settingmonitoringtransectinthemiddleandshallowwaterarea(altitude156-172m)inThreeGorgesreservoirhydro-fluctuationbelttoresearchthechangingcharacteristicsofthecontentsofN,P,K,pHandorganicmatterofthesoilwhichexperiencedtheinfluenceoffluctuationthefirsttime.Theresultsshowedthatbytheinfluenceofwaterlevelfluctuating,contentsofsoilN,P,K,pHandorganicmatterhadreducedindifferentsoillayersinhydro-fluctuationbelt.TheavailableNdecreasedby41.53%-59.87%,availablePdecreasedby5.26%-36.76%,availableKdecreasedby3.55%-45.56%,totalNdecreasedby9.52%-40.00%,totalPhadnochangegenerally,totalKhaddecreasedalittle,contentoforganicmaterialdecreasedby7.62%-37.83%%,pHvalueturnedtoneutral,changedby1.73%-9.58%.
简介:Theproteinstructureofthecellulosesynthase-likeprotein(CSL)wassimilartocellulosesynthase(CesA),includingtheconservativesequenceD,D,D,QXXRW.Onefull-lengthcDNAofthecellulosesynthase-likeproteinD(CslD)genewasclonedbyreversetranscriptase(RT)-polymerasechainreaction(PCR)with5’,3’rapidamplificationofcDNAends(RACE)methodsusingdegenerateprimersdesignedfromthehomologoussequencesoftheCesAgenes.Amultiplecomparisonsequenceanalysiswasconductedconcurrentlywithbioinformaticmethodstoanalyzetheobtainedsequence.ResultsofthesequenceanalysisshowedthatthiscDNAwas4150bpinlengthandcontainedasingleopenreadingframeencodingaproteinof1132aminoacids.Themultiplecomparisonsequenceanalysisshowedthatthededucedaminoacidsequencesharedhighsimilarity(over71%)withtheClCslDgenesfromPopulustremuloides,Oryzasativa,andArabidopsisthaliana.Thisworkwillhelplayanimportantfoundationforfurthermolecularstudieswithcellulosesynthesisofplants.