简介：Inrecentyears,heavyionbeamshavebeenrecognizedasaneffectiveandefficientphysicalmutagenduetotheircapacitytoinducemutationswithhighfrequencyandbroadspectrum.Nowadays,itisnotsodifficulttoproducevariousmutantsinplants.However,tominetheresponsiblemutatedgeneshasbeenanimportantchallenge.Formutationisolation,map-basedcloningisoneofthemajortraditionalwaystoisolatethemutantgenesthatcontroltraitsofinterestinforwardgeneticsstudies.However,theprocessofmap-basedcloningisusuallycomplicatedandtime-consuming.
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