简介:目的了解儿童急性呼吸道感染的病原体及其感染特点。方法选取某院2011年1—12月因急性呼吸道感染收治住院的患儿1410例,采用直接免疫荧光法检测7种病毒(包括副流感病毒I、Ⅱ、Ⅲ型,流感病毒A、B型,呼吸道合胞病毒和腺病毒),培养法检测细菌。分析不同月份、年龄段儿童病毒感染率及其合并细菌感染情况。结果1410份标本中,共检出病毒阳性594份(42.13%),以呼吸道合胞病毒和腺病毒为主;检出细菌阳性258份(18.30%)。3、4、10、11月份病毒检出率较高(均〉50%),7—9月病毒检出率较低(22.89%~31.40%);1~3岁组病毒检出率较高(58.04%),6~14岁组检出率较低(22.15%)。检出病毒混合感染阳性标本22份(1.56oA);单一病毒感染患儿合并细菌感染157例(27.45%)。流感病毒A、B型感染组合并细菌感染率较高,分别为47.06%、47.27%。结论儿童呼吸道病毒感染率与年龄和季节均相关;病毒感染后患儿合并细菌感染率较高,其中流感病毒A、B型感染更易合并细菌感染。
简介:Satellitecells(SCs)hasbeenshowntobethesourceofmyogenicprecursorcellsresponsibleformusclefiberrepairandregenerationduringatrophy,afterinjuryorneuromusculardisorders.RecentstudiesdemonstratedthefeasibilityofautologousSCstransplantationforrepairofmyocardialnecrosis.
简介:摘要目的分析解决TBA-40FR全自动生化分析仪中存在的问题。方法总结TBA-40FR全自动生化分析仪工作经验。结果总结出质控的若干问题及对策。结论使用TBA-40FR全自动生化分析仪的各个环节对检验质量都很重要。
简介:2008witnessesthe60thanniversaryofthefoundationofWorldHealthOrganization(WHO)andthe30thanniversaryoftheannouncementofAlmatyDeclaration.SponsoredbyWorldHealthOrganization,hostedbyChinaMinistryofHealthandChinaStateAdministrationofTraditionalChineseMedicineandco-organizedbyWFAS,WSMI,FIPandWFC,'2008WHOCongressonTraditionalMedicine'willbeheldinBeijingon7-9November2008,aimedatenhancingtheroleoftraditionalmedicine,reviewingtheachievementsmadebyassemblymembersinthisfield,andpromotingassemblycountriestointegratetraditionalmedicineintotheirnationalmedicalinsurancesystem,
简介:AbstractBackground:Electrical stimulation has been recommended as an effective therapy to prevent muscle atrophy after nerve injury. However, the effect of electrical stimulation on the proliferation of satellite cells in denervated muscles has not yet been fully elucidated. This study was aimed to evaluate the changes in satellite cell proliferation after electrical stimulation in nerve injury and to determine whether these changes are related to the restoration of myofiber cross-section area (CSA).Methods:Sciatic nerve crush injury was performed in 48 male Sprague-Dawley rats. In half (24/48) of the rats, the gastrocnemius was electrically stimulated transcutaneously on a daily basis after injury, while the other half were not stimulated. Another group of 24 male Sprague-Dawley rats were used as sham operation controls without injury or stimulation. The rats were euthanized 2, 4, and 6 weeks later. After 5-bromo-2’-deoxyuridine (BrdU) labeling, the gastrocnemia were harvested for the detection of paired box protein 7 (Pax7), BrdU, myofiber CSA, and myonuclei number per fiber. All data were analyzed using two-way analysis of variance and Bonferroni post-hoc test.Results:The percentages of Pax7-positive nuclei (10.81 ± 0.56%) and BrdU-positive nuclei (34.29 ± 3.87%) in stimulated muscles were significantly higher compared to those in non-stimulated muscles (2.58 ± 0.33% and 1.30 ± 0.09%, respectively, Bonferroni t = 15.91 and 18.14, P < 0.05). The numbers of myonuclei per fiber (2.19 ± 0.24) and myofiber CSA (1906.86 ± 116.51 μm2) were also increased in the stimulated muscles (Bonferroni t = 3.57 and 2.73, P < 0.05), and both were positively correlated with the Pax7-positive satellite cell content (R2 = 0.52 and 0.60, P < 0.01). There was no significant difference in the ratio of myofiber CSA/myonuclei number per fiber among the three groups.Conclusions:Our results indicate that satellite cell proliferation is promoted by electrical stimulation after nerve injury, which may be correlated with an increase in myonuclei number and myofiber CSA.