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简介：ObjectiveToinvestigateeffectoffeetorientationontheevaluationoftheposturalstabilityinpatientswithunilateralvestibularhypofunction(UVH)bytimedstandingtestsandstaticposturography(SPG).Methods65subjectswithUVHand92healthysubjectsregardedascontrolgrouptooktheposturalstabilitytestsinfourdifferentstancesincluding(1)standardRombergtest,(2)feet-apartstancetest,(3)tandemand(4)unilateralstandingtests.Ineachstance,theposturalstabilitywasmeasuredinbotheyesopen(EO)andeyesclosed(EC)conditions.Theaveragetimethatsubjectskeptbalancebeforefallingineachtestconditionswasrecordedbystopwatchasthetimedresult.Inaddition,theswayvelocity(SV)ofcenteroffootpressureintheuprightstanceduringstandardRombergtestandfeetapartstance,regardedasposturalstability,wasalsorecordedasSPG.Results(1)Thebalance-maintainingtimeoftheUVHgroupintandemandunilateralstandingwithEOandECwasdecreased(P<0.001)comparedwiththecontrolgroup.(2)TheSVsduringstandardRombergtestandfoot-apartstancewithEOwerenotdifferentbetweentheUVHgroupandcontrolgroup(P=0.118and0.110respectively),butsignificantwithECcondition(P<0.001).(3)Forbothgroups,theSVduringfoot-apartstandingwasshorterthanstandardRombergtestbothwithEOandEC(P<0.05andP<0.001).(4)TherewasnocorrelationbetweenthebalancetimeandSVforeithergroups(P>0.05).ConclusionsTheresultssuggestthatthetandemandunilateralstancetestsmayprovideadditionalinformationabouttheuprightstancetotheSPGmeasurementinpatientswithUVH.TheeffectoffeetorientationonSPGmeasurementsshouldbeconsidered.

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简介：AbstractBackground:Parkinson’s disease (PD) is the second most common neurodegenerative disease after Alzheimer’s dementia. Mitochondrial dysfunction is involved in the pathology of PD. Coiled-coil-helix-coiled-coil-helix domain-containing 2 (CHCHD2) was identified as associated with autosomal dominant PD. However, the mechanism of CHCHD2 in PD remains unclear.Methods:Short hairpin RNA (ShRNA)-mediated CHCHD2 knockdown or lentivirus-mediated CHCHD2 overexpression was performed to investigate the impact of CHCHD2 on mitochondrial morphology and function in neuronal tumor cell lines represented with human neuroblastoma (SHSY5Y) and HeLa cells. Blue-native polyacrylamide gel electrophoresis (PAGE) and two-dimensional sodium dodecyl sulfate-PAGE analysis were used to illustrate the role of CHCHD2 in mitochondrial contact site and cristae organizing system (MICOS). Co-immunoprecipitation and immunoblotting were used to address the interaction between CHCHD2 and Mic10. Serotype injection of adeno-associated vector-mediated CHCHD2 and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) administration were used to examine the influence of CHCHD2 in vivo.Results:We found that the overexpression of CHCHD2 can protect against methyl-4-phenylpyridinium (MPP+)-induced mitochondrial dysfunction and inhibit the loss of dopaminergic neurons in the MPTP-induced mouse model. Furthermore, we identified that CHCHD2 interacted with Mic10, and overexpression of CHCHD2 can protect against MPP+-induced MICOS impairment, while knockdown of CHCHD2 impaired the stability of MICOS.Conclusion:This study indicated that CHCHD2 could interact with Mic10 and maintain the stability of the MICOS complex, which contributes to protecting mitochondrial function in PD.

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