简介:比较支持者天赋杀手(A-NK)的增长和cytotoxicity之间的差别与没有浆液的中等AIMV和标准有教养的房间在vitro的包含浆液的媒介,并且也在激活和IL-2-treatedA-NK房间的形态学特性上观察IL-12的帮助效果,细胞的增长被MTT方法在vitro评估。A-NK房间打死的目标房间的形态学通过验电器被观察。所有能很快在没有浆液的中等AIMV有教养的A-NK房间增殖并且在标准包含浆液的媒介与那相比使cytotoxicity高。中等剂量的IL-2和IL-12激活的A-NK房间比高剂量的IL-2-treatedA-NK房间优异。这些数据显示没有浆液的中等AIMV能为culturingA-NK房间代替标准包含浆液的媒介,并且中等剂量的IL-2和IL-12能减少高剂量的IL-2引起的副作用。学习为A-NK房间的试验性、临床的准备提供了一个新试验性的基础。
简介:Tostudytheroleofnaturalkiller(NK)cellsinTcellrecruitmentinmurineliverinfectedwithvirus,micewereintravenouslyinjecteddailywithanti-NK1.1^+antibodytodepleteNKcells.Lymphocytesinthelivertissueofmiceinfectedwithtype5adenovirusdepletedintheE1andE3regionswereassessedbyfluorometricactivatedcellsorting(FACS).Ex-pressionofchemokineIP-10anditsreceptorCXCR3mRNAintheliver,hepaticlymphocytesandspleentissuewereexaminedbyreversetranscriptionpolymerasechainreaction(RT-PCR).Serumalmfineaminotransferase(ALT)wasmeasuredasanindicatorofliverinjury.Itwasfoundthatinfectionofadenovimsandanfi-Fasmonoclonalantibody(mAb)intomicecausedliverinjuryandhighexpressionofinterfemn-γinducibleprotein-10(IP-10)mRNAintheliver.Anfi-NK1.1^+mAb,whichwasintraperitoneallyinjectedintothemiceinfectedwithadenovirus,suppressesTcellrecruitmentandexpressionofIP-10mRNAinthehver.Slighterhverinjurywasalsoobserved.Afterviresinfection,expressionofCXCR3mRNAinspleenandhvertissuewasobservedatdifferenttime.TheresultssuggestedthatTcellrecruitmentwasinitiatedbyNKcelldependentchemokineIP-10,whichinducedactivatedTcellspriminginthespleentothehverofthemouse.NKcellsplayedakeyroleinTcellrecruitmentintheliverofmouseinfectedwithadenovims.
简介:摘要目的由于颅后窝的解剖特点,颅后窝血肿发生率低,其中以硬膜外血肿最多见。我院自2001-2009年共收治颅后窝硬膜外血肿病人33例。现对此病诊治报告如下。
简介:在在最后十年的免疫学的最重要的进步是对规章的淋巴细胞,在Treg之中,房间和规章的NKT房间对不仅免疫学者而且几乎所有生物医学的研究人员吸引人的描述。同时,NK房间是“杀手”而且调整天生、适应的免疫,这被注意不仅特别在早舞台,由secreting,cytokines和房间房间联系。在这评论,我们将简短包括T房间在规章的淋巴细胞总结进步(Treg,Tr1,Th3),NKT房间和NK房间,然后广泛地在两正常有免疫力的回答并且处于疾病状况介绍NK房间的积极规章的功能(肿瘤,感染和autoimmunity),并且最后,在正常、病原的有免疫力的反应上在NK房间的否定规章的效果集中于最最近的前进。在结论,我们推测那“规章的NK(NK-reg)”房间子集存在并且需要探索。细胞与分子的免疫学。2006;3(4):241-254。
简介:GuidedbyTCMtreatmentonthebasisofsyndromedifferentiation,theauthorhastreated33casesofhysteromyoma,whicharedividedintostagnationofliver-qiandbloodstasis(SLQBS)type,andqi-deficiencyandbloodstasis(QDBS)type,byacupuncture-moxibus-tionandTuina.After3therapeuticcourses,thetotalef-fectiverateis81.8%.ThetherapeuticeffecthasnocloserelationshipwiththeTCMsyndromedifferentiation.
简介:ToexploretheeffectofrhlL-15onCB-CD34^+stemcellscommittingtoNKcells,CD34^+stemcellswereobtainedfromcordblood(CB)bymagnetic-assistedcellsorting(MACS)method.CD3,CD16andCD56moleculesexpressedoncellsurfaceweredetectedbyflowcytometer.MTFmethodwasusedtotestthecytotoxicityofNKcells.Theresultswerethatstemcellfactor(SCF)alonehasnoeffectonCD34^+stemcells.IL-15stimulatedCD34^+stemcellscommittoNKcells,andSCFshowedstrongsynergisticeffectwithIL-15.ItwasconcludedthatIL-15andSCFplayeddifferentrolesduringNKcelldevelopment,llr15promotedCD34^+stemcellsdifferentiatetoNKcellprecursorandSCFimprovedtheeffectsofIL-15onNKcelldifferentiation.