简介:Qian-Zheng-San,atraditionalChineseprescriptionconsistingofTyphoniiRhizoma,BombyxBatryticatus,Scorpio,hasbeenfoundtoplayanactivetherapeuticroleincentralnervoussystemdiseases.However,itisunclearwhetherQian-Zheng-Sanhastherapeuticvalueforperipheralnerveinjury.Therefore,weusedSprague-Dawleyratstoinvestigatethis.Asciaticnervecrushinjurymodelwasinducedbyclampingtherightsciaticnerve.Subsequently,ratsinthetreatmentgroupwereadministered2mLQian-Zheng-San(1.75g/mL)dailyassystemictherapyfor1,2,4,or8weeks.RatsinthecontrolgroupwerenotadministeredQian-Zheng-San.Ratsinshamgroupdidnotundergosurgeryandsystemictherapy.Footprintanalysiswasusedtoassessnervemotorfunction.Electrophysiologicalexperimentswereusedtodetectnerveconductionfunction.Immunofluorescencestainingwasusedtoassessaxoncountsandmorphologicalanalysis.Immunohistochemicalstainingwasusedtoobservemyelinregenerationofthesciaticnerveandthenumberofmotoneuronsintheanteriorhornofthespinalcord.At2and4weekspostoperatively,thesciaticnervefunctionindex,nerveconductionvelocity,thenumberofdistantregeneratedaxonsandtheaxondiameterofthesciaticnerveincreasedintheQian-Zheng-Santreatmentgroupcomparedwiththecontrolgroup.At2weekspostoperatively,nervefiberdiameter,myelinthickness,andthenumberofmotorneuronsinthelumbarspinalcordanteriorhornincreasedintheQian-Zheng-Santreatmentgroupcomparedwiththecontrolgroup.TheseresultsindicatethatQian-Zheng-Sanhasapositiveeffectonperipheralnerveregeneration.
简介:Sheng-Mai-San(SMS),awell-knownChinesemedicinalplantformula,iswidelyusedforthetreatmentofcardiacdiseasescharacterizedbydeficiencyofQiandYinsyndrome.Amousechronicintermittenthypoxia(CIH)modelwasestablishedtomimictheprimaryclinicalfeaturesofdeficiencyofQiandYinsyndrome.MiceexperiencedCIHfor28days(nadir7%topeak8%oxygen,20minperday),resultinginleftventricle(LV)dysfunctionandstructureabnormalities.AfteradministrationofSMS(0.55,1.1,and5.5g·kg-1·d-1)forfourweeks,improvedcardiacfunctionwasobserved,asindicatedbytheincreaseintheejectionfractionfromtheLVonechocardiography.SMSalsopreservedthestructuralintegrityoftheLVagainsteccentrichypotrophy,tissuevacuolization,andmitochondrialinjuryasmeasuredbyhistology,electronmicroscopy,andultrasoundassessments.Mechanistically,theantioxidanteffectsofSMSweredemonstrated;SMSwasabletosuppressmitochondrialapoptosisasindicatedbythereductionofseveralpro-apoptoticfactors(Bax,cytochromec,andcleavedcaspase-3)andup-regulationoftheanti-apoptosisfactorBcl-2.Inconclusion,theseresultsdemonstratethatSMStreatmentcanprotectthestructureandfunctionoftheLVandthattheprotectiveeffectsofthisformulaareassociatedwiththeregulationofthemitochondrialapoptosispathway.
简介:Thepresentstudywasdesignedtoestablishamulti-wavelengthquantitativefingerprintingmethodforSan-HuangTablets(SHT),awidelyusedandcommerciallyavailableherbalpreparation,wherehighperformanceliquidchromatography(HPLC)withadiodearraydetector(DAD)wasemployedtoobtainthefingerprintprofiles.Asimplelinearquantitativefingerprintmethod(SLQFM)coupledwithmulti-ingredientsimultaneousdeterminationwasdevelopedtoevaluatethequalityconsistencyofthetestedsamplesqualitativelyandquantitatively.Additionally,thecomponent–activityrelationshipbetweenchromatographicfingerprintsandtotalradical-scavengingcapacityinvitro(asassessedusingthe1,1-diphenyl-2-picrylhydrazyl(DPPH)assay)wasinvestigatedbypartialleastsquaresregression(PLSR)analysistopredicttheantioxidantcapacityofnewsamplesfromthechromatographicfingerprintsandidentifythemainactiveconstituentsthatcanbeusedasthetargetmarkersforthequalitycontrolofSHT.Inconclusion,thestrategydevelopedinthepresentstudywaseffectiveandreliable,whichcanbeemployedforholisticevaluationandaccuratediscriminationforthequalityconsistencyofSHTpreparationsandothertraditionalChinesemedicine(TCM)andherbalpreparationsaswell.
简介:摘要目的对使用3MFiltekTMZ350和登仕柏光固化复合树脂充填前牙邻面龋的疗效进行临床观察和对比方法临床选择422颗患牙分为实验组和对照组各211颗,实验组使用3MZ350充填,对照组使用登仕柏复合树脂充填。按照各自产品说明书的步骤进行窝洞处理、黏结、充填、固化和磨光,经过半年、1年、2年后观察疗效。结果两种树脂的继发龋、边缘密合性、边缘着色、表明粗糙度、色泽匹配、牙龈健康状况Z在1年左右实验组优于对照组。结论Z350光固化复合树脂充填前牙不仅满足了病患的美观要求,避免了冠修复造成的牙体损伤,同时大大降低了病人的经济负担。
简介:摘要目的对比分析直接树脂充填法和间接树脂充填法修复后牙病理性磨损的临床效果。方法对我院近期收治的60例后牙病理性磨损的患者为研究对象,并随机分组,分为治疗组30例与对照组30例,治疗组给予间接的树脂充填修复法,对照组给予直接的树脂充填修复法,比较治疗1年后树脂充填的临床效果及咀嚼效率的情况。结果治疗1年后复查,间接法组患牙树脂充填体A级率优于直接法组,两组差异有统计学意义(P<0.05)。治疗3个月及治疗1年后,间接法组的咀嚼效率高于直接法,两组差异有统计学意义(P<0.05)。结论间接树脂法比直接树脂充填法修复后牙病理性磨损有较好的临床效果以及咀嚼效率,值得临床上的广泛推广使用。