简介：BackgroundTheregulationoft-PAgeneistheessenceandcoreofthrombosis.Therefore,thepresentstudyaimedtopreparenanot-PAgenecoatedstentandtoobserveitseffectoncoronarystentthrombosisindogs.MethodsHighlyexpressedt-PAgeneplasmidwasconstructedandalbuminnanot-PAgenecoatingstentwasprepared.Themajorbranchvesselsofdogcoronaryarterywerepre-expandedwitha3.0mm×20balloonwith8-10atmosphericpressure.10dogsofthecontrolgroupwereimplantedwithbaremetalstent;while12dogsoftheexperimentalgroupwereimplantedwithnanot-PAgenecoatingstent.Bothgroupswerenotgivenanti-coagulationtreatments.Bloodsamplesweretakenfort-PAandD-dimerbeforetheoperation,at1,2,4and8weeksafteroperation.Pathologicalanalysisfoundthrombosisinthecavityandthehyperplasiaoftheintima.t-PAexpressionwasdetectedbyimmunohistochemicalindirectly,andthethicknessoftheintimaofthesectioncrosswasdirectlymeasuredbymorphometry.Liver,heart,kidneysandlungweretakenforpathologicalobservation.ResultsAllexperimentalanimalssurvivedatpostoperative8weeks.Vascularstentthrombosiswasseenin10casesofthecontrolgroupwiththethrombosisrateof100%;whilewasseenin2casesamong12casesoftheexperimentalgroupwiththethrombosisratewas16.67%(P=0.00087).Immunohistochemicalstainingshowedthatthepositivet-PAgenetransfectionoftheexperimentalgroupwasmainlydistributedonthesurfaceofhyperplasiaintima,andvascularwallt-PAexpressionofthecontrolgroupwasnegative.Positivet-PAsignalwasnotfoundintheliver,heart,kidneysandlung.ConclusionNanot-PAgenevectorcoatingstentcaneffectivelyexpresst-PAinvascularwallandeffectivelypreventsstentthrombosis.

简介：为了描述创造登陆的一种新奇技术，在trabecular网状组织以内脱衣指导trabecular微绕过stent(iStent)在经历了phacoemulsification.METHODSThirty的病人的培植--从从2014年5月在phacoemulsification以后经历了iStent培植到2015年2月的30个病人的四只眼睛在我们的回顾的学习被包括。所有iStents经由登陆长带技术被植入。A25计量器microvitreoretinal片被用来切分trabecular网状组织到不到1钟小时，有效地创造登陆脱衣。iStent棉棍沿着登陆长带被压然后stent被释放进34与iStent培植看的trabecularmeshwork.RESULTSOf，(79.4%)27看有的主要开角度的绿内障，(17.6%)6看有的pseudoexfoliation绿内障，并且(2.9%)1看有的眼睛的高血压。在6月的后续(n=17)，低血压患者药的吝啬的数字从2.2减少了