简介:Thelight-harvestingchlorophylla/b-proteincomplexplaysanimportantroleinphotosynthesisofplants.Afull-lengthcDNAoflight-harvestingchlorophylla/b(cab)genewasclonedfromthefirststrandofMoso(Phyllostachysedulis)cDNAthroughRT-PCRandRACEmethods,namedascab-PhE10(cabgene10fromPh.edulis).Thelengthofcab-PhE10(GenBankaccessionnumber:EU118754)is1151bp,whichcontainsanopenreadingframeencoding283aminoacidsfrom81stto932ndposition.Thebioinformaticsanalysisindicatedthattheproteinencodedbycab-PhE10hadachlorophlla/bbindingdomain(83rd-247thposition),twoproteinkinaseC-phosphorylationsites,threeN-myristoylationsitesandayiaA/Bdoublehelixdomain.Theaminoacidsequenceofcab-PhE10showedhighsimilaritywiththecabgenesofOryzasativa,Zeamays,Hordeumvulgare,andVitisvinifera,morethan80%,respectively,whichindicatedthatcab-PhE10genebelongstolhcb5genefamily.
简介:Weusedgeographicinformationsystemapplicationsandstatisticalanalysestoclassifyyoung,prematureforestareasinsoutheasternGeorgiausingcombineddatafromLandsatTM5satelliteimageryandgroundinventorydata.Wedefinedprematurestandsasforestswithtreesupto15yearsold.Weestimatedtheprematureforestareasusingthreemethods:maximumlikelihoodclassification(MLC),regressionanalysis,andk-nearestneighbor(kNN)modeling.Overallaccuracy(OA)ofclassifyingtheprematureforestusingMLCwas82%andtheKappacoefficientofagreementwas0.63,whichwasthehighestamongthemethodsthatwehavetested.ThekNNapproachrankedsecondinaccuracywithOAof61%andaKappacoefficientofagreementof0.22.RegressionanalysisyieldedanOAof57%andaKappacoefficientof0.14.WeconcludethatLandsatimagerycanbeeffectivelyusedforestimatingprematureforestareasincombinationwithimageprocessingclassifierssuchasMLC.
简介:PUBs蛋白调控了作物的生长发育及响应非生物胁迫和生物胁迫的过程。为探讨棉花中U-box类型泛素连接酶家族,本研究利用生物信息学方法分析了二倍体雷蒙德氏棉中PUBs的数目、进化、基因结构、结构域分布及基因表达模式。结果表明,雷蒙德氏棉中有93个GrPUBs基因家族成员,基因长度为1101~11191bp。亚细胞定位预测结果表明,GrPUBs编码产物大部分定位在细胞质和细胞核中,少数定位在胞外基质线粒体中。根据除U-box以外所含结构域将该家族成员分为7类,分别含有UFD2结构域、ARM结构域、激酶结构域、只含U-box、WD-40、TPR以及异构酶结构域。GrPUBs基因家族染色体定位显示,基因在13条染色体上均有分布,但分布不均匀。在第5条染色体最多,有11条基因序列,第4、第12和Scaffold染色体上的基因最少,仅有2条。基因结构分析表明,基因内含子的数目变化较大,在0~17之间,且具有相似结构的基因,其编码蛋白聚为一类。基因表达模式分析发现。几乎1/3的基因在花中优势表达,个别基因在开花后10d、20d、30d和40d的种子中表达量高于在叶和花中的表达,如Gorai.006G251300。本试验为深入研究U-box类型泛素连接酶在棉花生长发育及抗逆的机理提供了理论指导。