简介:Phospholipidsareamajorkindoflipidsinricegrainsandhavefundamentalnutritionalandfunctionalbenefitstotheplant.Theirlysoforms(lysophospholipids,LPLs)oftenforminclusioncomplexeswithamyloseorindependentlyinfluencethephysicochemicalandfunctionalpropertiesofricestarch.However,thegeneticbasisforLPLsynthesisinriceendospermislargelyunknown.Here,weperformedapreliminaryassociationtestof13LPLcompositionsamong20riceaccessions,andidentified22putativemain-effectquantitativetraitlociresponsibleforallLPLsexceptforLPC14:0andLPE14:0.Fivederivedcleavedamplifiedpolymorphicsequencesandoneinsertion/deletionmarkerforthreeLPL-synthesis-relatedcandidategenesweredeveloped.AssociationanalysisrevealedtwomarkerssignificantlyassociatedwithstarchLPLtraits.Theseresultsprovideaninsightintothegeneticbasisofphospholipidbiosynthesisinriceandmaycontributetothericequalitybreedingprogramsusingfunctionalmarkers.